Zhou Yiyan, Wang Feiran, Zhao Rui, Huang Tianyi, Su Wentao, Wen Ya, He Zhixian, Li Dong
Medical School of Nantong University, Nantong 226001, China; Department of Thyroid and Breast Surgery, Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong, China.
Department of Thyroid and Breast Surgery, Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong, China.
Food Res Int. 2025 Nov;219:117011. doi: 10.1016/j.foodres.2025.117011. Epub 2025 Jul 15.
Food nutrition and safety are fundamental to the food industry, and the development of appropriate research models is crucial. Unlike traditional animal models, the innovative organoid/organ-on-a-chip model possess distinct human-like characteristics and genomic stability, which have garnered significant attention in food research. In this review, we conduct a comparative analysis between organoids and traditional animal and 2D cell models. We subsequently conduct a comprehensive and systematic assessment of the applications of organoid/organ-on-a-chip technology across diverse areas of food research. This evaluation encompasses investigations into foodborne pathogens, functional food factors, toxicology, flavor perception, and artificial foods. While organoids have successfully addressed several limitations inherent in traditional models, they still face challenges in fully replicating the in vivo microenvironment. Moving forward, the development of organoid/organ-on-a-chip systems in food research should take into account the complexity of biological processes, the importance of integrating multiple systems, and the need for standardized measurements. The innovative advancement of organoid technology is anticipated to provide a robust theoretical foundation for future research in food, food nutrition and safety.
食品营养与安全是食品工业的基础，开发合适的研究模型至关重要。与传统动物模型不同，创新的类器官/芯片上器官模型具有独特的类人特征和基因组稳定性，在食品研究中受到了广泛关注。在本综述中，我们对类器官与传统动物模型和二维细胞模型进行了比较分析。随后，我们对类器官/芯片上器官技术在食品研究各个领域的应用进行了全面系统的评估。该评估涵盖了对食源性病原体、功能性食品因子、毒理学、风味感知和人造食品的研究。虽然类器官已经成功解决了传统模型固有的一些局限性，但它们在完全复制体内微环境方面仍面临挑战。展望未来，食品研究中类器官/芯片上器官系统的发展应考虑生物过程的复杂性、整合多个系统的重要性以及标准化测量的需求。类器官技术的创新进展有望为未来食品、食品营养与安全研究提供坚实的理论基础。
