Huang Yuting, Wu Xiaoyan, Xu Yongxin, Yang Nengjie, Xi Peipei, Wang Yunan, Zhu Yujuan, Chen Xiaodong
Department of Dermatology, Research Center of Immunology, Affiliated Hospital of Nantong University, Medical School of Nantong University, No. 19 Qixiu Road, Xinchengqiao Street, Chongchuan District, Nantong 226001, China.
Department of Rheumatology, Research Center of Immunology, Affiliated Hospital of Nantong University, Medical School of Nantong University, No. 19 Qixiu Road, Xinchengqiao Street, Chongchuan District, Nantong 226001, China.
Burns Trauma. 2025 May 3;13:tkaf029. doi: 10.1093/burnst/tkaf029. eCollection 2025.
As the largest organ in the human body, the skin protects the body from pathogens and harmful substances through physical, chemical, and immune barrier functions. However, accurately replicating the complex physiology of human skin in mouse models remains a significant challenge. Accurately replicating the complex physiology of human skin in mouse models remains a significant challenge, making the development of bionic artificial skin particularly important. In recent years, skin organoid and skin-on-a-chip technologies have greatly enhanced skin modeling, overcoming many limitations of traditional approaches. In this review, we comprehensively summarize important advances in research on skin organoids and skin-on-a-chip. First, we present the anatomical structures and functional roles of the different skin layers. We then highlight current construction techniques and research findings on skin organoids and skin-on-a-chip. We then discuss in detail the biomedical applications of these emerging technologies. However, current models of skin organoids and skin-on-a-chip still have limitations. Therefore, we summarize the key challenges and explore strategies to improve the complexity and maturation of skin models via the precise control over the microenvironment. In the future, with the advancement of bioengineering technology, skin organoids, and skin-on-a-chip will provide more powerful tools for skin disease research and treatment.
作为人体最大的器官，皮肤通过物理、化学和免疫屏障功能保护身体免受病原体和有害物质的侵害。然而，在小鼠模型中准确复制人类皮肤的复杂生理机能仍然是一项重大挑战。在小鼠模型中准确复制人类皮肤的复杂生理机能仍然是一项重大挑战，这使得仿生人工皮肤的开发尤为重要。近年来，皮肤类器官和芯片上皮肤技术极大地推动了皮肤建模，克服了传统方法的许多局限性。在这篇综述中，我们全面总结了皮肤类器官和芯片上皮肤研究的重要进展。首先，我们介绍了不同皮肤层的解剖结构和功能作用。然后，我们重点介绍了当前皮肤类器官和芯片上皮肤的构建技术及研究成果。接着，我们详细讨论了这些新兴技术的生物医学应用。然而，目前的皮肤类器官和芯片上皮肤模型仍存在局限性。因此，我们总结了关键挑战，并探索通过精确控制微环境来提高皮肤模型的复杂性和成熟度的策略。未来，随着生物工程技术的进步，皮肤类器官和芯片上皮肤将为皮肤疾病的研究和治疗提供更强大的工具。
