Barroso Tiago Linhares Cruz Tabosa, Castro Luiz Eduardo Nochi, da Cruz Elder Pacheco, Ferreira Vanessa Cosme, da Rosa Rafael Gabriel, Goldbeck Rosana, Forster-Carneiro Tânia
Faculdade de Engenharia de Alimentos (FEA), Universidade Estadual de Campinas (UNICAMP), Rua Monteiro Lobato, 80, 13083-862, Campinas, São Paulo, Brazil.
Faculdade de Engenharia de Alimentos (FEA), Universidade Estadual de Campinas (UNICAMP), Rua Monteiro Lobato, 80, 13083-862, Campinas, São Paulo, Brazil.
Food Res Int. 2025 Nov;219:117019. doi: 10.1016/j.foodres.2025.117019. Epub 2025 Jul 25.
The hydrolysis of biomass in fermentative processes often faces the difficulty of generating inhibitory products. Its reduction or removal is essential to enable the use of agro-industrial waste, such as cashew apple bagasse. Therefore, this study aimed to find an optimized condition for the hydrolysis of cashew apple bagasse by subcritical water and to introduce an in-line pre-purification process. The semicontinuous process conditions varied in temperature (80-220 °C) and solid quantity (4-11 g), maintaining a flow of 5 mL min, 150 MPa, acidified with HPO at pH 2.0 for 30 min. The analyses were carried out for sugar yield, total phenolic compounds (TPC), furanic aldehydes, and organic acids. Structural and calorimetric analyses were also performed to evaluate the effects on the biomass after hydrolysis. The characterization of solids revealed changes in the biomass, indicating effective removal of hemicellulose and structural alterations. The results demonstrated that a higher temperature and a mass of 7.5 g enabled the achievement of a higher sugar yield (641.13 mg g). This condition was tested in-line with an activated carbon adsorption column at 40-80 °C. A 36 % reduction in TPC at 40 °C minimized sugar loss to 5.72 % while removing 23 % of organic acids from the hydrolysate. This discovery was crucial to increasing the viability of biomass for fermentation processes. Integrating purification stages represented a promising strategy to improve the quality of hydrolysates for industrial applications, contributing to biomass valorization.
发酵过程中生物质的水解常常面临产生抑制性产物的难题。减少或去除这些抑制性产物对于利用农业工业废弃物（如腰果苹果渣）至关重要。因此，本研究旨在寻找亚临界水水解腰果苹果渣的优化条件，并引入在线预纯化工艺。半连续工艺条件在温度（80 - 220℃）和固体量（4 - 11克）方面有所变化，保持流速为5毫升/分钟，压力为150兆帕，用磷酸在pH 2.0条件下酸化30分钟。对糖产量、总酚化合物（TPC）、呋喃醛和有机酸进行了分析。还进行了结构和量热分析，以评估水解后对生物质的影响。固体表征显示生物质发生了变化，表明半纤维素被有效去除且结构发生了改变。结果表明，较高的温度和7.5克的质量能够实现更高的糖产量（641.13毫克/克）。此条件在40 - 80℃下与活性炭吸附柱进行在线测试。在40℃时，总酚化合物减少36%，糖损失最小化至5.72%，同时从水解产物中去除了23%的有机酸。这一发现对于提高生物质用于发酵过程的可行性至关重要。整合纯化阶段是提高水解产物质量以用于工业应用的一种有前景的策略，有助于生物质的增值。
