Kaur Jaspreet, Taggar Monica Sachdeva, Kalia Anu, Sanghera Gulzar Singh, Khatkar Sunil Kumar, Vashisht Pranav, Singh Lovepreet
Department of Biochemistry, Punjab Agricultural University, Ludhiana, India.
Department of Renewable Energy Engineering, Punjab Agricultural University, Ludhiana, India.
Environ Technol. 2025 Jul;46(17):3363-3373. doi: 10.1080/09593330.2025.2464265. Epub 2025 Feb 16.
For the extraction of sugars and their subsequent conversion into ethanol, removing lignin from sugarcane bagasse is a major challenge attributed to its recalcitrant nature. This study compares the efficacy of green deep eutectic solvents with conventional acid/alkali pre-treatments for the delignification of sugarcane bagasse. Among different deep eutectic solvent pre-treatments, the maximum removal of lignin i.e. 77.37% was reported when bagasse was treated with choline chloride: formic acid (1:2) for 6 h. The comparison between deep eutectic solvents and conventional acid/alkali pretreatments revealed that acid (HSO) pre-treatment showed no significant reduction in lignin content. However, the alkaline pre-treatment with 1 M NaOH for 60 min resulted in significant removal of lignin content (83.17%) from bagasse compared to deep eutectic solvent pre-treatment. Fourier transform infrared spectroscopy and scanning electron microscopic results of bagasse indicated significant structural alterations after the pre-treatment. The saccharification of alkali-pretreated bagasse with in-house cellulase resulted in a maximum reducing sugar concentration of 54.50 g/L with a hydrolytic efficiency of 67.01%. The batch fermentation of bagasse hydrolysate with resulted in an ethanol concentration of 9.55 g/L with a fermentation efficiency of 53.81%. This study made a median attempt to identify an effective pre-treatment method to delignifying sugarcane bagasse, ultimately enhancing the enzymatic accessibility and increasing the efficiency of cellulose hydrolysis into fermentable sugars.
为了提取糖类并将其随后转化为乙醇，从甘蔗渣中去除木质素是一项重大挑战，这归因于其顽固的性质。本研究比较了绿色深共熔溶剂与传统酸/碱预处理对甘蔗渣脱木质素的效果。在不同的深共熔溶剂预处理中，当甘蔗渣用氯化胆碱：甲酸（1:2）处理6小时时，报告的木质素最大去除率为77.37%。深共熔溶剂与传统酸/碱预处理之间的比较表明，酸（HSO）预处理的木质素含量没有显著降低。然而，与深共熔溶剂预处理相比，用1 M NaOH进行60分钟的碱性预处理导致甘蔗渣中木质素含量显著去除（83.17%）。甘蔗渣的傅里叶变换红外光谱和扫描电子显微镜结果表明预处理后结构发生了显著变化。用自制纤维素酶对碱预处理的甘蔗渣进行糖化，得到的最大还原糖浓度为54.50 g/L，水解效率为67.01%。甘蔗渣水解产物的分批发酵得到乙醇浓度为9.55 g/L，发酵效率为53.81%。本研究进行了一次中等程度的尝试，以确定一种有效的预处理方法来使甘蔗渣脱木质素，最终提高酶的可及性并提高纤维素水解为可发酵糖的效率。
