Karatug Kacar Ayse, Ertik Onur, Dinckurt Nilay, Arabaci Unal, Obakan Yerlikaya Pinar, Yanardag Refiye, Bolkent Sehnaz
Department of Biology, Faculty of Science, Istanbul University, Istanbul, Türkiye.
Department of Chemistry, Faculty of Science and Engineering, Bursa Technical University, Bursa, Türkiye.
J Food Sci. 2025 Sep;90(9):e70544. doi: 10.1111/1750-3841.70544.
Diabetes is a metabolic and chronic disease affecting different tissues' metabolism. Genetic factors, lifestyles, and dietary habits can cause it. In diabetes, oxidative stress can occur in metabolic disorders, negatively affecting it. The antioxidants are essential in reducing or completely stopping the harmful effects of these adverse effects on the tissues. In the present study, we aimed to determine the combined effects of lipoic acid, vitamin E, and selenium in the kidneys of diabetic mice. For this experiment, the Balb/c mice were used and divided into five groups: citrate buffer, the solvents of the antioxidants, combined the antioxidants (α-lipoic acid, vitamin E, and selenium), streptozotocin, combined with the antioxidants and streptozotocin (A+D). At the end of 30 days of this process, the mice were sacrificed by cervical dislocation. Kidney tissues were taken for morphological, Western blotting, and biochemical analyses. The tissue was used for staining with Masson's trichrome and periodic acid-Schiff (PAS) of renal tissue sections taken for histological analysis; Western blotting such as the level of IL-10, IL-1β, TGF-β, p38, cCas3, NRF2; biochemical parameters such as the level of GSH, LPO, SOD, CAT, GR, TAS, TOS, ROS, OSI, PON, CA, LDH, AR, ADA, arginase, OH-proline, and AOPP. The histological findings showed mild damage to the kidney tissue of diabetic mice. Western blot results showed that the damage was independent of inflammation. Biochemical results revealed that administering combined antioxidants to diabetic mice protects the kidney tissue.
糖尿病是一种影响不同组织代谢的慢性代谢性疾病。遗传因素、生活方式和饮食习惯均可导致该病。在糖尿病中，氧化应激可发生于代谢紊乱过程中，并对其产生负面影响。抗氧化剂对于减轻或完全阻止这些不良反应对组织的有害影响至关重要。在本研究中，我们旨在确定硫辛酸、维生素E和硒对糖尿病小鼠肾脏的联合作用。为此实验，选用Balb/c小鼠并将其分为五组：柠檬酸盐缓冲液（抗氧化剂的溶剂）、抗氧化剂组合（α-硫辛酸、维生素E和硒）、链脲佐菌素、抗氧化剂与链脲佐菌素组合（A+D）。在此过程30天结束时，通过颈椎脱臼法处死小鼠。取肾脏组织进行形态学、蛋白质印迹法和生化分析。该组织用于对肾组织切片进行Masson三色染色和过碘酸希夫（PAS）染色以进行组织学分析；蛋白质印迹法检测如IL-10、IL-1β、TGF-β、p38、cCas3、NRF2的水平；生化参数检测如GSH、LPO、SOD、CAT、GR、TAS、TOS、ROS、OSI、PON、CA、LDH、AR、ADA、精氨酸酶、羟脯氨酸和晚期氧化蛋白产物（AOPP）的水平。组织学结果显示糖尿病小鼠的肾脏组织有轻度损伤。蛋白质印迹结果表明该损伤与炎症无关。生化结果显示给糖尿病小鼠施用联合抗氧化剂可保护肾脏组织。
