Jacklyn C L, Matte S J, Ryder D I, Cousins C L, Moss M A
Clin Biochem. 1985 Dec;18(6):342-4. doi: 10.1016/s0009-9120(85)80072-2.
We have evaluated the precision of the Ektachem 400 thin film enzymic creatinine method and compared it to that obtained for an AutoAnalyzer II (AAII) picric acid procedure. The results of the study show that the Ektachem method yields imprecise creatinine values over the low concentration range (40-240 mumol/L). Run-to-run precision estimates at a creatinine concentration of 225 mumol/L (by AAII) yielded CV's of 9.3 and 2.6% for the Ektachem 400 and AutoAnalyzer II methods respectively. The Ektachem 400 yielded imprecise creatinine determinations even when sample ammonia concentrations were less than 150 mumol/L. Patient samples analyzed by the AAII and Ektachem 400 over the creatinine range 40-900 mumol/L were highly correlated (r = 0.994). The correlation (r = 0.779) was low for patient data over the creatinine range 40-160 mumol/L. Creatinine results determined by the Ektachem 400 showed improved precision when samples were evaluated in triplicate using Kodak's latest (No. 8.5) software revision.
我们评估了Ektachem 400薄膜酶法测定肌酐的精密度，并将其与自动分析仪II（AAII）苦味酸法的精密度进行了比较。研究结果表明，Ektachem法在低浓度范围（40 - 240μmol/L）内得出的肌酐值不精确。在肌酐浓度为225μmol/L（通过AAII测定）时，Ektachem 400法和自动分析仪II法的批间精密度估计值的变异系数（CV）分别为9.3%和2.6%。即使样本氨浓度低于150μmol/L，Ektachem 400法得出的肌酐测定结果仍不精确。通过AAII和Ektachem 400对肌酐浓度在40 - 900μmol/L范围内的患者样本进行分析，结果高度相关（r = 0.994）。对于肌酐浓度在40 - 160μmol/L范围内的患者数据，相关性（r = 0.779）较低。当使用柯达最新版（第8.5版）软件对样本进行三次重复评估时，Ektachem 400测定的肌酐结果显示出更高的精密度。
