对柯达埃克塔化学400分析仪进行了评估，用于血浆肌酐的自动化酶法测定。

The Kodak Ektachem 400 Analyzer evaluated for automated enzymic determination of plasma creatinine.

作者信息

Smith C H, Landt M, Steelman M, Ladenson J H

出版信息

Clin Chem. 1983 Jul;29(7):1422-5.

Abstract

We have evaluated an automated creatinine iminohydrolase procedure for plasma creatinine analysis, as used in the Kodak Ektachem 400 Analyzer. The correlation with kinetic and end-point (equilibrium) alkaline picrate procedures is generally excellent. The enzymatic procedure has the advantage of being unaffected by bilirubin, cephalosporins, and acetoacetate, all of which interfere in alkaline picrate procedures. Its accuracy, however, greatly depends on calibration values provided by the manufacturer. Storage of samples at -20 degrees C produced little change in measured creatinine. The Ektachem procedure has a greater range of linearity (140 mg/L) than the alkaline picrate procedures, but it shares with them the disadvantages of suboptimal precision in the normal range. The increased specificity, ease of performance, and other advantages of this use of creatinine iminohydrolase make it an attractive procedure.

摘要

我们评估了一种用于血浆肌酐分析的自动化肌酐亚氨基水解酶检测方法，该方法用于柯达Ektachem 400分析仪。与动力学和终点（平衡）碱性苦味酸盐检测方法的相关性总体良好。酶法的优点是不受胆红素、头孢菌素和乙酰乙酸的影响，而这些物质都会干扰碱性苦味酸盐检测方法。然而，其准确性很大程度上取决于制造商提供的校准值。将样品储存在-20℃时，测得的肌酐变化很小。Ektachem检测方法的线性范围（140mg/L）比碱性苦味酸盐检测方法更大，但它与碱性苦味酸盐检测方法一样，在正常范围内存在精密度欠佳的缺点。肌酐亚氨基水解酶的这种应用具有更高的特异性、操作简便等优点，使其成为一种有吸引力的检测方法。