Melo Radamés Bezerra, Costa Gabriella Alves Julião, de Barros Silva Paulo Goberlânio, Oriá Reinaldo Barreto, de Aquino Cristhyane Costa, Vasconcelos Paulo Roberto Leitão
Department of Dentistry, Paulo Picanço School of Dentistry, Fortaleza, Ceará, Brazil.
Department of Dentistry, Unichristus, Fortaleza, Ceará, Brazil.
Inflammopharmacology. 2025 Sep 9. doi: 10.1007/s10787-025-01892-x.
This study evaluated the effects and mechanisms of antioxidant and anti-inflammatory oils with a high omega-9:omega-6 ratio and a low omega-6:omega-3 ratio on post-extraction healing in rats.
A total of 128 Wistar rats were divided into four groups: Sham, Saline, Isolipidic, and Anti-inflammatory/Antioxidant. The animals received one of the following treatments: (1) 0.9% NaCl solution (Sham Group or Saline Group); (2) an isolipidic mixture containing alpha-linolenic acid (ALA) and a combination of ω-9, ω-6, and ω-3 fatty acids derived from corn oil and soybean oil-rich in omega-6:omega-3 and omega-9:omega-6 ratios (Isolipidic Group); or (3) an anti-inflammatory and antioxidant mixture containing ALA, docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA), composed of olive oil (61%), canola oil (24%), and fish oil (15%) (Test Group). Treatments were administered by gavage for four days pre-extraction and three days post-surgery. Animals were euthanized at 3, 7, 14, and 21 days post-extraction. Blood and tissue samples were analyzed for myeloperoxidase, osteoclast count, IL-1β, NFκB, iNOS, BMP-2 expression, glutathione (GSH), and thiobarbituric acid reactive substances (TBARS).
The Anti-inflammatory/Antioxidant mixture significantly reduced myeloperoxidase expression, osteoclast count, GSH, TBARS, and IL-1β levels on day 7. It also decreased NFκB, iNOS, and BMP-2 expression on days 3 and 7 compared to the Saline and Isolipidic groups.
The Anti-inflammatory and Antioxidant mixture effectively reduced inflammation and oxidative stress during the inflammatory and proliferative phases of post-extraction healing, suggesting potential benefits in clinical applications for oral surgery recovery.
本研究评估了具有高ω-9:ω-6比例和低ω-6:ω-3比例的抗氧化和抗炎油对大鼠拔牙后愈合的影响及机制。
总共128只Wistar大鼠被分为四组：假手术组、生理盐水组、等脂组和抗炎/抗氧化组。动物接受以下处理之一：(1) 0.9%氯化钠溶液（假手术组或生理盐水组）；(2) 一种等脂混合物，含有α-亚麻酸（ALA）以及源自玉米油和富含ω-6:ω-3和ω-9:ω-6比例的大豆油的ω-9、ω-6和ω-3脂肪酸组合（等脂组）；或(3) 一种抗炎和抗氧化混合物，含有ALA、二十二碳六烯酸（DHA）和二十碳五烯酸（EPA），由橄榄油（61%）、菜籽油（24%）和鱼油（15%）组成（试验组）。在拔牙前4天和手术后3天通过灌胃给予处理。在拔牙后3、7、14和21天对动物实施安乐死。分析血液和组织样本中的髓过氧化物酶、破骨细胞计数以及白细胞介素-1β（IL-1β）、核因子κB（NFκB）诱导型一氧化氮合酶（iNOS）、骨形态发生蛋白-2（BMP-2）表达、谷胱甘肽（GSH）和硫代巴比妥酸反应性物质（TBARS）。
抗炎/抗氧化混合物在第7天显著降低了髓过氧化物酶表达、破骨细胞计数、GSH、TBARS和IL-1β水平。与生理盐水组和等脂组相比，在第3天和第7天它还降低了NFκB iNOS和BMP-2表达。
抗炎和抗氧化混合物在拔牙后愈合的炎症和增殖阶段有效减轻了炎症和氧化应激，提示在口腔外科手术恢复的临床应用中具有潜在益处。
