Rosenblum P M, Yamada L, Callard I P, Callard G V
Comp Biochem Physiol B. 1985;82(4):659-65. doi: 10.1016/0305-0491(85)90504-8.
Highly specific antisera for 11-keto- and 11 beta-hydroxytestosterone have been raised in sheep. Assay systems for the simultaneous measurement of 11-ketotestosterone, 11 beta-hydroxytestosterone, testosterone, progesterone and estradiol-17 beta were validated for Ictalurus nebulosus plasma and Carassius auratus serum. In males of both species 11-ketotestosterone and testosterone were the major steroids detected. In females, testosterone and estradiol-17 beta were the predominant steroids measured. Data from samples taken at different stages of the annual cycle suggest that seasonal fluctuations in gonadal steroid secretion occur in I. nebulosus and C. auratus.
已在绵羊体内培育出针对11-酮睾酮和11β-羟基睾酮的高特异性抗血清。用于同时测量11-酮睾酮、11β-羟基睾酮、睾酮、孕酮和雌二醇-17β的检测系统已在斑点叉尾鮰血浆和鲫鱼血清中得到验证。在这两个物种的雄性中，检测到的主要类固醇是11-酮睾酮和睾酮。在雌性中，测量到的主要类固醇是睾酮和雌二醇-17β。来自在年度周期不同阶段采集的样本的数据表明，斑点叉尾鮰和鲫鱼的性腺类固醇分泌存在季节性波动。
