Schlaghecke R, Kley H K, Krüskemper H L
J Clin Chem Clin Biochem. 1986 Aug;24(8):577-81. doi: 10.1515/cclm.1986.24.8.577.
A radioimmunoassay (RIA) is described for the measurement of 11 beta,17 beta-dihydroxy-4-androsten-3-one (11 beta-hydroxytestosterone) in human plasma. The reliability criteria of the new RIA were similar to those of other steroid hormone radioimmunoassays. The mean plasma 11 beta,17 beta-dihydroxy-4-androsten-3-one level for healthy young subjects was 1.31 +/- 0.32 nmol/l (means +/- SD) in males and 1.19 +/- 0.35 nmol/l in females at 8 a.m.; during the night, there was a marked decrease, and at 11 p.m. the recorded values were 0.41 +/- 0.15 nmol/l and 0.46 +/- 0.14 nmol/l, respectively. During the corticotropin stimulation test, 11 beta,17 beta-dihydroxy-4-androsten-3-one increased from 1.03 +/- 0.33 nmol/l to 1.31 +/- 0.41 nmol/l, while in dexamethasone suppression tests a decrease from 2.04 +/- 0.82 nmol/l to 0.25 +/- 0.05 nmol/l was seen. In contrast, chorionic gonadotropin administration on 3 consecutive days did not influence plasma concentrations of 11 beta,17 beta-dihydroxy-4-androsten-3-one.
本文描述了一种用于测定人血浆中11β,17β-二羟基-4-雄烯-3-酮（11β-羟基睾酮）的放射免疫分析法。新放射免疫分析法的可靠性标准与其他类固醇激素放射免疫分析法相似。健康年轻男性受试者上午8点时血浆中11β,17β-二羟基-4-雄烯-3-酮的平均水平为1.31±0.32nmol/L（均值±标准差），女性为1.19±0.35nmol/L；夜间该水平显著下降，晚上11点时记录值分别为0.41±0.15nmol/L和0.46±0.14nmol/L。在促肾上腺皮质激素刺激试验中，11β,17β-二羟基-4-雄烯-3-酮从1.03±0.33nmol/L升至1.31±0.41nmol/L，而在地塞米松抑制试验中则从2.04±0.82nmol/L降至0.25±0.05nmol/L。相比之下，连续3天注射绒毛膜促性腺激素对11β,17β-二羟基-4-雄烯-3-酮的血浆浓度没有影响。
