Hu Xiaohao, Liang Anru, Zhao Tongling, Ling Yu, Wei Yanlin, Zuo Yuer, Li Hongmian
Department of Emergency, The People's Hospital of Guangxi Zhuang Autonomous Region and Research Center of Medical Sciences, Guangxi Academy of Medical Sciences, Nanning, Guangxi, China.
Department of Plastic Surgery, The Second Nanning People's Hospital and The third Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
PLoS One. 2025 Sep 9;20(9):e0330078. doi: 10.1371/journal.pone.0330078. eCollection 2025.
Radiotherapy, a prevalent and effective treatment for various malignancies, often causes collateral damage to normal skin and soft tissues in the irradiated area. To address this, we developed a novel approach combining SVFG-modified adipose-derived high-activity matrix cell clusters (HAMCC) with concentrated growth factors (CGF) to enhance regeneration and repair of radiation-induced skin and soft tissue injuries. Our study included cellular assays, wound healing evaluations, and histological analyses. In the inflammatory wound healing environment, this treatment reduced ROS and 8-oxo-dG expression while increasing four antioxidant-related proteins (Nrf2, HO-1, NQO1, AKR1C1), thereby suppressing oxidative stress and improving wound healing efficiency. Additionally, the HAMCC and CGF combination promoted collagen expression and rearrangement and accelerated angiogenesis. This innovative treatment presents a promising strategy for regenerating and repairing radiation-induced skin and soft tissue damage.
放射疗法是治疗各种恶性肿瘤的一种常用且有效的方法，但它常常会对受照射区域的正常皮肤和软组织造成附带损伤。为了解决这个问题，我们开发了一种新方法，将经基质血管片段（SVFG）修饰的脂肪源性高活性基质细胞簇（HAMCC）与浓缩生长因子（CGF）相结合，以增强放射性皮肤和软组织损伤的再生与修复能力。我们的研究包括细胞实验、伤口愈合评估和组织学分析。在炎性伤口愈合环境中，这种治疗方法降低了活性氧（ROS）和8-氧代脱氧鸟苷（8-oxo-dG）的表达，同时增加了四种与抗氧化相关的蛋白质（Nrf2、血红素氧合酶-1（HO-1）、醌氧化还原酶1（NQO1）、醛酮还原酶1C1（AKR1C1）），从而抑制氧化应激并提高伤口愈合效率。此外，HAMCC与CGF的组合促进了胶原蛋白的表达和重排，并加速了血管生成。这种创新治疗方法为放射性皮肤和软组织损伤的再生与修复提供了一种有前景的策略。
