Zhang Lixiang, Yuan Zhengchao, Shafiq Muhammad, Cai Youjun, Wang Zewen, Nie Piming, Mo Xiumei, Xu Yuan
Department of Orthopaedics, Xinqiao Hospital, Army Military Medical University, No. 183, Xinqiao Street, Shapingba District, Chongqing, 400037, China.
State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, Shanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Biological Science and Medical Engineering, Donghua University, Shanghai, 201620, China.
Macromol Biosci. 2023 Apr;23(4):e2200500. doi: 10.1002/mabi.202200500. Epub 2023 Feb 22.
Growth factors are essential for wound healing owing to their multiple reparative effects. Concentrated growth factor (CGF) is a third-generation platelet extract containing various endogenous growth factors. Herein, a CGF extract solution is combined with gelatin methacrylate (GM) by physical blending to produce GM@CGF hydrogels for wound repair. The GM@CGF hydrogels show no immune rejection during autologous transplantation. Compared to CGF, GM@CGF hydrogels not only exhibit excellent plasticity and adhesivity but also prevent rapid release and degradation of growth factors. The GM@CGF hydrogels display good injectability, self-healing, swelling, and degradability along with outstanding cytocompatibility, angiogenic functions, chemotactic functions, and cell migration-promoting capabilities in vitro. The GM@CGF hydrogel can release various effective molecules to rapidly initiate wound repair, stimulate the expressions of type I collagen, transform growth factor β1, epidermal growth factor, and vascular endothelial growth factor, promote the production of granulation tissues, vascular regeneration and reconstruction, collagen deposition, and epidermal cell migration, as well as prevent excessive scar formation. In conclusion, the injectable GM@CGF hydrogel can release various growth factors and provide a 3D spatial structure to accelerate wound repair, thereby providing a foundation for the clinical application and translation of CGF.
生长因子因其多种修复作用而对伤口愈合至关重要。浓缩生长因子（CGF）是一种含有多种内源性生长因子的第三代血小板提取物。在此，通过物理混合将CGF提取液与甲基丙烯酸明胶（GM）相结合，制备用于伤口修复的GM@CGF水凝胶。GM@CGF水凝胶在自体移植过程中无免疫排斥反应。与CGF相比，GM@CGF水凝胶不仅具有优异的可塑性和粘附性，还能防止生长因子的快速释放和降解。GM@CGF水凝胶在体外表现出良好的可注射性、自愈性、膨胀性和降解性，以及出色的细胞相容性、血管生成功能、趋化功能和促进细胞迁移的能力。GM@CGF水凝胶可释放多种有效分子，迅速启动伤口修复，刺激I型胶原蛋白、转化生长因子β1、表皮生长因子和血管内皮生长因子的表达，促进肉芽组织生成、血管再生与重建、胶原蛋白沉积以及表皮细胞迁移，同时防止过度瘢痕形成。总之，可注射的GM@CGF水凝胶能够释放多种生长因子，并提供三维空间结构以加速伤口修复，从而为CGF的临床应用和转化奠定基础。
