Liu Yongjian, Han Chenyang, Guo Li, Li Wenyan, Wu Shasha, Sheng Jian, Zhai Liping, Shen Heping, Wang Genghuan
Interventional Department, First Affiliated Hospital of Dalian Medical University, Dalian, China.
The Second Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang, China.
CNS Neurosci Ther. 2025 Sep;31(9):e70569. doi: 10.1111/cns.70569.
To investigate the effects and mechanisms of S-adenosylmethionine (SAM) from deer antler on improving depression-like behaviors in chronic unpredictable mild stress (CUMS) mice.
The CUMS method was used to establish a mouse depression model. The relationship between SAM and HIF-1α was analyzed by small molecule-protein docking and molecular dynamics simulation.
Network pharmacology analysis showed that the effect of SAM is related to HIF-1α. When shRNA-HIF-1α was used to silence the expression of HIF-1α, the effect of SAM was inhibited. The improvement of depression-like behaviors in mice was not significant, and inflammatory factors were no longer further downregulated.
SAM can improve depression-like behaviors in CUMS mice and enhance their mobility. SAM inhibits neuroinflammation and glycolysis, and also suppresses the activation of microglia.
探讨鹿茸中S-腺苷甲硫氨酸（SAM）改善慢性不可预测性轻度应激（CUMS）小鼠抑郁样行为的作用及机制。
采用CUMS法建立小鼠抑郁模型。通过小分子-蛋白质对接和分子动力学模拟分析SAM与低氧诱导因子-1α（HIF-1α）之间的关系。
网络药理学分析表明，SAM的作用与HIF-1α有关。当使用shRNA-HIF-1α沉默HIF-1α表达时，SAM的作用受到抑制。小鼠抑郁样行为的改善不明显，炎症因子也不再进一步下调。
SAM可改善CUMS小鼠的抑郁样行为并增强其活动能力。SAM抑制神经炎症和糖酵解，还可抑制小胶质细胞的激活。
