De Bisschop H C, Van Driessche E E, Alberty M L, Willems J L
Fundam Appl Toxicol. 1985 Dec;5(6 Pt 2):S175-9.
The degradation of soman in human plasma and at a physiological pH has been studied at the high nanomolar level. From a comparison between degradation of racemic soman in preincubated and unpreincubated plasma the concentration of phosphonylation sites was estimated at some 120 nM. The contribution of phosphonylation to the degradation of soman is eliminated by preincubation of the plasma with racemic soman. The rate constants of the total hydrolysis (enzymatic and nonenzymatic) were calculated from the semi-log plot of the degradation of C(+)- or C(-)-soman in preincubated plasma/Tris (50% v/v). The nonenzymatic part of the hydrolysis was estimated from experiments in plasma ultrafiltrate (100,000 NMWL)/Tris (50% v/v). The nonenzymatic contribution to the hydrolysis of the P(+)-isomers is negligible compared to the enzymatic hydrolysis.
已在高纳摩尔水平研究了梭曼在人血浆及生理pH值条件下的降解情况。通过比较外消旋梭曼在预孵育血浆和未预孵育血浆中的降解情况，估计膦酰化位点的浓度约为120 nM。通过将血浆与外消旋梭曼预孵育，消除了膦酰化对梭曼降解的影响。根据预孵育血浆/Tris（50% v/v）中C(+)-或C(-)-梭曼降解的半对数图，计算了总水解（酶促和非酶促）的速率常数。通过在血浆超滤物（100,000 NMWL）/Tris（50% v/v）中的实验估计了水解的非酶促部分。与酶促水解相比，非酶促对P(+)-异构体水解的贡献可忽略不计。
