Saint-André S, Désiré B
C R Acad Sci III. 1985;301(3):67-72.
Soman (pinacolyl methylphosphonofluoridate), a mixture of four stereoisomers, is inactivated appreciably in Tris buffer, pH 7.40, mu = 0.155 at 25 degrees C by beta-cyclodextrin (cycloheptaamylose, beta-CD). Under these conditions, the dissociation constant Kd of the 1:1 complex formed by beta-CD and soman and the rate constant k2 for the phosphonylation of beta-CD by soman are (0.53 +/- 0.05)mM and (5.9 +/- 0.6) X 10(-2) min-1 respectively. It results that the inactivation of soman by the mono-anion of beta-CD is about 2,600 times faster than the hydrolysis of soman by the hydroxide ion. The inactivation of both P(-) isomers of soman by beta-CD proceeds apparently at the same rate but both P(+) isomers react more slowly. Thus the interaction is stereospecific. The inactivation of soman by beta-CD appears to be as fast in human plasma in vitro as in Tris buffer.
梭曼（频哪基甲基膦酰氟）是四种立体异构体的混合物，在25℃、pH值为7.40、离子强度μ = 0.155的Tris缓冲液中，可被β-环糊精（环庚糖，β-CD）显著灭活。在这些条件下，β-环糊精与梭曼形成的1:1复合物的解离常数Kd以及梭曼对β-环糊精进行膦酰化反应的速率常数k2分别为(0.53 ± 0.05)mM和(5.9 ± 0.6)×10⁻² min⁻¹。结果表明，β-环糊精的单阴离子对梭曼的灭活作用比氢氧根离子对梭曼的水解作用快约2600倍。β-环糊精对梭曼的两种P(-)异构体的灭活作用显然以相同速率进行，但两种P(+)异构体的反应较慢。因此，这种相互作用具有立体特异性。在体外，β-环糊精对梭曼的灭活作用在人血浆中似乎与在Tris缓冲液中一样快。
