基于Q Exactive液相色谱-串联质谱代谢组学对两种肥胖综合征代谢物差异的初步研究

Preliminary study on metabolite differences between two obesity syndromes based on Q Exactive liquid chromatography-tandem mass spectrometry metabolomics.

作者信息

Jing Ye, Xu Xiaoxuan, Wang Yanhong, Qu Xue, Guo Yunlong, Guo Ao, Dai Yulin, Liu Yangyang, Yue Hao

机构信息

Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, China.

Department of College of Traditional Chinese Medicine, Changchun University of Chinese Medicine, Changchun, China.

出版信息

PLoS One. 2025 Sep 10;20(9):e0331901. doi: 10.1371/journal.pone.0331901. eCollection 2025.

DOI:10.1371/journal.pone.0331901

PMID:40929178

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12422424/

Abstract

BACKGROUND

According to traditional Chinese medicine, based on the pathogenesis, clinical manifestation, and treatment, obesity can be classified into two broad types, namely, "shi obesity" and "xu obesity." The aim of this study was to explore the differences in metabolite levels between these two types of obesity.

METHODS

Q Exactive liquid chromatography-tandem mass spectrometry was used to analyze the metabolites in the sera of 30 healthy adults, 30 adults with shi obesity, and 30 adults with xu obesity. Possible metabolic pathways associated with biological alterations were identified using the Human Metabolome Database and Kyoto Encyclopedia of Genes and Genomes analyses.

RESULTS

Fifteen important potential biomarkers were identified, 14 of which were upregulated. Furthermore, the biomarkers phenylalanine, L-tyrosine, glycerophosphocholine, and LysoPC(O-18:0/0:0) were significantly correlated with both shi and xu obesity. Of note, L-tyrosine and glycerophosphocholine were screened and used as potential biomarkers of shi and xu obesity for the first time.

CONCLUSION

This study verified the differences in serum metabolites associated with shi and xu obesity, demonstrating potential therapeutic strategies for the two types.

摘要