Ruci Mamica, Kongoli Renata, Coppola Francesca, Succi Mariantonietta, Testa Bruno, Kyçyk Onejda, Karaulli Julian, Lamçe Fatbardha, Iorizzo Massimo
Faculty of Biotechnology and Food, Food Research Center, Agricultural University of Tirana, Tirana, Albania.
Department of Agro-food Technology, Faculty of Biotechnology and Food, Agricultural University of Tirana, Tirana, Albania.
Front Microbiol. 2025 Aug 26;16:1654308. doi: 10.3389/fmicb.2025.1654308. eCollection 2025.
Non- wine yeasts have a promising role in biotechnological approaches to enhance wine complexity, particularly by influencing the aromatic profile. is a non- yeast that is notable for its antimicrobial activity and diverse enzymatic activities. These properties make this yeast a valid candidate for application as a starter culture in winemaking. This study evaluated the application of in sequential inoculation with for the production of Kallmet wine, delivered from the indigenous Kallmet grape variety traditionally cultivated in various regions of Albania. The use of different inoculation strategies resulted in significant differences in wine composition, affecting key oenological parameters, the aromatic profile and sensory attributes. 62 did not negatively interfere with the fermentation kinetics of during fermentation and contributed to wines with lower amounts of ethanol and richer in glycerol, total polyphenols and anthocyanins. Moreover, increased levels of isobutanol, phenylethyl alcohol, isoamyl alcohol and monoterpenes (linalool, geraniol, and nerol) were observed in wines produced with 62 and in sequential inoculation, compared to wines fermented solely with . Finally, sensory analysis revealed a distinct differentiation in the wines, attributable to the modulation of aromatic compounds by 62.
非酿酒酵母在提升葡萄酒复杂度的生物技术方法中具有重要作用,尤其是在影响香气特征方面。某非酿酒酵母以其抗菌活性和多种酶活性而闻名。这些特性使这种酵母成为葡萄酒酿造中作为起始培养物应用的有效候选者。本研究评估了该非酿酒酵母与酿酒酵母顺序接种在阿尔巴尼亚各地传统种植的本土卡尔梅特葡萄品种酿造卡尔梅特葡萄酒中的应用。使用不同的接种策略导致葡萄酒成分存在显著差异,影响了关键的酿酒参数、香气特征和感官属性。该非酿酒酵母在发酵过程中未对酿酒酵母的发酵动力学产生负面影响,并有助于酿造出乙醇含量较低、甘油、总多酚和花青素含量较高的葡萄酒。此外,与仅用酿酒酵母发酵的葡萄酒相比,在顺序接种该非酿酒酵母和酿酒酵母所酿造的葡萄酒中,异丁醇、苯乙醇、异戊醇和单萜类化合物(芳樟醇、香叶醇和橙花醇)的含量有所增加。最后,感官分析表明,由于该非酿酒酵母对芳香化合物的调节作用,葡萄酒之间存在明显差异。