el-Yazbi F A, Korany M A, Bedair M
J Clin Hosp Pharm. 1985 Dec;10(4):373-7. doi: 10.1111/j.1365-2710.1985.tb00936.x.
A simple and rapid colorimetric method for the determination of imipramine hydrochloride and desipramine hydrochloride in their tablet formulations and in biological fluids is presented. The method is based on the reaction of these drugs with 3-methyl-2-benzothiazolone hydrazone in the presence of ferric chloride, with direct measurement at 635 nm. Cyclohexane was used to extract these drugs from serum and urine, at basic pH, by a single manual extraction. The method can detect 0.5 microgram/ml of each drug. The main advantages of this method are its simplicity and high sensitivity.
本文介绍了一种简单快速的比色法，用于测定片剂制剂和生物流体中的盐酸丙咪嗪和盐酸地昔帕明。该方法基于这些药物在氯化铁存在下与3-甲基-2-苯并噻唑酮腙的反应，在635nm处直接测量。在碱性pH值下，通过单次手动萃取，用环己烷从血清和尿液中萃取这些药物。该方法可检测每种药物0.5微克/毫升。该方法的主要优点是简单和高灵敏度。
