Ogawa H I, Nakamura M
J Gen Microbiol. 1985 Nov;131(11):3117-26. doi: 10.1099/00221287-131-11-3117.
A new Mycoplasmatales virus, referred to as MV-O1, was isolated during cloning of Acholeplasma oculi 19L. The virus formed plaques only on strains of A. oculi, i.e. the original clone, A. oculi 19L, a subclone of A. oculi 19L (A.oculi-i), A. oculi Goat 5 and wild isolate (K-2) of A. oculi, but not on other acholeplasmas, including strains of A. laidlawii, nor on five human mycoplasma species tested. The virus required horse serum for multiplication as well as for plaque formation and passed through a 100 nm filter. Electron microscopy revealed enveloped, spherical particles 80-130 nm in diameter. The buoyant density of purified virus was 1.23 g ml-1 in CsCl, and agarose gel electrophoresis indicated that the viral nucleic acid was DNA.
在眼支原体19L的克隆过程中分离出一种新的支原体病毒，称为MV - O1。该病毒仅在眼支原体菌株上形成噬菌斑，即原始克隆眼支原体19L、眼支原体19L的一个亚克隆（眼支原体 - i）、眼支原体山羊5以及眼支原体的野生分离株（K - 2），但在其他支原体上不形成噬菌斑，包括莱氏无胆甾原体菌株，在测试的五种人支原体物种上也不形成噬菌斑。该病毒增殖以及形成噬菌斑都需要马血清，并且能通过100纳米滤器。电子显微镜显示病毒粒子为有包膜的球形，直径80 - 130纳米。纯化病毒在氯化铯中的浮力密度为1.23克/毫升，琼脂糖凝胶电泳表明病毒核酸是DNA。
