Jwanny E W, Rashad M M
J Basic Microbiol. 1985;25(10):645-51. doi: 10.1002/jobm.3620251009.
The utilization of methanol by Candida lipolytica was studied with respect to the biomass, protein, lipid and other metabolites contents. Supplementation of the methanol medium with certain redox agents such as 2,6-dichlorophenol indophenol, reduced glutathione and methyl red, showed a stimulation of both growth and protein production of C. lipolytica. At certain growth conditions the cellular yield of C. lipolytica was 0.3 g of cells/g of methanol and the protein of the biomass was 41.0%. The amino acid composition of the protein especially of the essential ones was comparable to FAO standards. Thin-layer chromatography of the lipids indicated a dominance of total glycerides. Analyses of the acylated unsaponified fraction by gas liquid chromatography showed that campesterol was the major component.
研究了解脂假丝酵母利用甲醇的情况，涉及生物量、蛋白质、脂质和其他代谢产物的含量。在甲醇培养基中添加某些氧化还原试剂，如2,6-二氯酚靛酚、还原型谷胱甘肽和甲基红，结果表明对解脂假丝酵母的生长和蛋白质生产均有促进作用。在某些生长条件下，解脂假丝酵母的细胞产量为每克甲醇产生0.3克细胞，生物量中的蛋白质含量为41.0%。该蛋白质的氨基酸组成，尤其是必需氨基酸的组成，与粮农组织标准相当。脂质的薄层色谱分析表明总甘油酯占主导地位。通过气液色谱对酰化未皂化部分的分析表明，菜油甾醇是主要成分。
