与蜂毒蜂毒肽复合的Ca2+ -钙调蛋白晶体的X射线晶体学和色谱表征。

X-ray crystallographic and chromatographic characterization of the crystals of Ca2+-calmodulin complexed with bee venom melittin.

作者信息

Tanaka Y, Takahashi S, Mitsui Y, Itoh S, Iitaka Y, Kasai H, Okuyama T

出版信息

J Mol Biol. 1985 Dec 5;186(3):675-7. doi: 10.1016/0022-2836(85)90141-x.

Abstract

Crystals of calmodulin complexed with both Ca2+ and melittin, a peptide from bee venom, have been grown from 2-methyl-2,4-pentanediol solution by using the hanging drop method of vapour diffusion. The crystals belong to space group P2(1)2(1)2(1) with a = 97.3(9) A, b = 56.5(0) A, c = 33.4(9) A and Z = 4. Analyses of the dissolved crystals by high performance liquid chromatography show that the crystals contain a 1:1 complex of calmodulin and melittin. An asymmetric unit contains one such complex and the solvent content of the crystals is 47.5% (v/v).

摘要

已通过气相扩散悬滴法从2-甲基-2,4-戊二醇溶液中培养出与钙离子和蜂毒肽（一种来自蜂毒的肽）复合的钙调蛋白晶体。这些晶体属于空间群P2(1)2(1)2(1)，a = 97.3(9) Å，b = 56.5(0) Å，c = 33.4(9) Å，Z = 4。通过高效液相色谱对溶解的晶体进行分析表明，晶体包含钙调蛋白和蜂毒肽的1:1复合物。一个不对称单元包含一个这样的复合物，晶体的溶剂含量为47.5%（体积/体积）。