Lee Yeonji, Kim Suhyeok, Hasanthi Mirasha, Song Sanghyun, Kim Sua, Lee Kyeong-Jun
Department of Marine Life Science, Jeju National University, Jeju 63243, Republic of Korea.
Department of Marine Life Science, Jeju National University, Jeju 63243, Republic of Korea; Marine Science Research Institute, Jeju National University, Jeju 63333, Republic of Korea.
Comp Biochem Physiol B Biochem Mol Biol. 2025 Sep 10;281:111162. doi: 10.1016/j.cbpb.2025.111162.
This research aimed to determine the dietary requirement of thiamine and evaluate its effects on olive flounder (Paralichthys olivaceus). Control diet (Con) was formulated without thiamine supplementation, while four additional diets were formulated with thiamine concentrations of 4, 8, 12 and 16 mg/kg diet (designated as T4, T8, T12 and T16, respectively). A total of 300 fish (initial weight, 55.5 ± 1.3 g) were randomly distributed into 15 tanks (triplicate groups per treatment, 20 fish per tank) and fed for 10 weeks. One-way ANOVA was performed to analyze treatment effects. Final body weight was significantly increased in all the thiamine-supplemented groups compared to the Con group. Significant improvements in weight gain, specific growth rate and feed utilization were observed in T4 and T16 groups compared to the Con group. Immune responses, including anti-protease, lysozyme and myeloperoxidase activities and total immnunoglobulin concentration were significantly increased in the T4-T12 thiamine-supplemented groups. Antioxidant activity was significantly increased in all thiamine-supplemented groups. Trypsin activity was significantly elevated in the 4-12 mg/kg groups, while amylase and lipase activities were enhanced in all thiamine-supplemented groups. Anti-inflammatory genes were significantly upregulated in thiamine-supplemented groups compared to the Con. Thiamine supplementation promoted an increase in goblet cell count and villi length in intestine. A broken-line regression analysis for weight gain indicated that the optimal thiamine requirement for olive flounder would be 3.05 mg/kg diet. These findings demonstrate the positive effects of dietary thiamine on growth, immunity, antioxidant activity, digestive enzyme activity and intestinal health in olive flounder.
本研究旨在确定硫胺素的日粮需求量，并评估其对牙鲆（Paralichthys olivaceus）的影响。对照日粮（Con）在配制时未添加硫胺素，而另外四种日粮分别添加了浓度为4、8、12和16 mg/kg日粮的硫胺素（分别指定为T4、T8、T12和T16）。总共300尾鱼（初始体重，55.5±1.3 g）被随机分配到15个水箱中（每个处理设三个重复组，每个水箱20尾鱼），并投喂10周。采用单因素方差分析来分析处理效果。与Con组相比，所有添加硫胺素的组最终体重均显著增加。与Con组相比，T4和T16组的体重增加、特定生长率和饲料利用率有显著改善。在添加硫胺素的T4-T12组中，免疫反应，包括抗蛋白酶、溶菌酶和髓过氧化物酶活性以及总免疫球蛋白浓度显著增加。所有添加硫胺素的组抗氧化活性均显著增加。4-12 mg/kg组的胰蛋白酶活性显著升高，而所有添加硫胺素的组淀粉酶和脂肪酶活性均增强。与Con组相比，添加硫胺素的组抗炎基因显著上调。硫胺素添加促进了肠道杯状细胞数量和绒毛长度的增加。对体重增加进行折线回归分析表明，牙鲆的最佳硫胺素需求量为3.05 mg/kg日粮。这些发现证明了日粮硫胺素对牙鲆生长、免疫、抗氧化活性、消化酶活性和肠道健康具有积极影响。
