da Silva Jeanderson Marcelino, da Silva Wesly Mikael, da Silva Meykson Alexandre, de Lima José Rivaldo, de Lima Júnior Nelson Correia, de Santana Jonata Henrique, Fernandes Mariana Pinheiro, da Silva Teresinha Gonçalves, Chagas Cristiano Aparecido, de Aguiar Júnior Francisco Carlos Amanajás, Ferraz Navarro Daniela Maria do Amaral, Galdino Dos Santos Fábio Henrique, de Oliveira Alves João Victor, da Silva Márcia Vanusa, Correia Maria Tereza Dos Santos
Programa de Pós-Graduação em Ciências Biológicas, Centro de Biociências, Universidade Federal de Pernambuco, Recife, PE, 50670-901, Brazil.
Departamento de Química, Universidade Federal da Paraíba, João Pessoa, PB, 58033-455, Brazil.
J Ethnopharmacol. 2025 Sep 10;355(Pt A):120588. doi: 10.1016/j.jep.2025.120588.
Syagrus vagans, known popularly as Ariri, is a palm found in the Caatinga, whose seed oil has been utilized therapeutically to relieve various health conditions. However, ethnopharmacological research on its efficacy and safety remains scarce.
The study aimed to describe fatty acid profile of S. vagans fixed oil (SvO), as well as toxicological evaluation and its pharmacological activities, including antigenotoxicity, antimutagenicity, antinociceptive, and anti-inflammatory.
Initially, the fixed oil was analyzed by GC-FID. HCT-116 and RAW 264.7 cell lines were seeded in microplates with SvO, and cell viability was evaluated through the MTT colorimetric method. Acute toxicity, genotoxicity, and mutagenicity were assessed after the administration of a single dose of SvO (2000 mg/kg per os) in mice. SvO was also evaluated for antigenotoxicity and antimutagenicity in a model of methyl methanesulfonate (MMS)-induced genetic damage in vivo. The antinociceptive and anti-inflammatory properties of SvO were evaluated by abdominal writhing, formalin, carrageenan-induced paw edema, and peritonitis tests conducted in mice.
The fatty acid profile of SvO revealed that the major components were lauric acid, palmitic acid, and linoleic acid. The IC of SvO for HCT-116 and RAW 264.7 exceeded 200 μg/mL and 196.8 μg/mL, respectively. Acute toxicity, genotoxicity, and mutagenicity assessments in mice revealed no mortality, behavioral changes, biochemical alterations, histopathological damage to organs, or impacts on genetic material. SvO protected the DNA from damage induced by MMS. There was a dose-dependent reduction in the number of abdominal writhings induced by acetic acid, as well as a decreased licking duration in the first and second phases of the formalin test. Furthermore, SvO demonstrated an anti-edematogenic effect in carrageenan-induced edema and a significant decrease in the migration of neutrophils, leukocytes, IL-1β, and TNF-α in the peritonitis assay.
Ingestion of SvO proved to be safe, protected DNA from damage induced by methyl methanesulfonate, as well as exhibited antinociceptive and anti-inflammatory effects.
Syagrus vagans，俗称Ariri，是一种生长在卡廷加地区的棕榈树，其种子油已被用于治疗各种健康问题。然而，关于其功效和安全性的民族药理学研究仍然很少。
本研究旨在描述Syagrus vagans固定油（SvO）的脂肪酸谱，以及其毒理学评估和药理活性，包括抗基因毒性、抗诱变、抗伤害感受和抗炎作用。
首先，通过气相色谱-火焰离子化检测器（GC-FID）分析固定油。将HCT-116和RAW 264.7细胞系接种于含有SvO的微孔板中，通过MTT比色法评估细胞活力。在小鼠口服单剂量SvO（2000 mg/kg）后，评估急性毒性、遗传毒性和诱变性。还在体内甲磺酸甲酯（MMS）诱导的遗传损伤模型中评估SvO的抗基因毒性和抗诱变作用。通过小鼠腹部扭体试验、福尔马林试验、角叉菜胶诱导的足趾肿胀试验和腹膜炎试验评估SvO的抗伤害感受和抗炎特性。
SvO的脂肪酸谱显示主要成分是月桂酸、棕榈酸和亚油酸。SvO对HCT-116和RAW 264.7的半数抑制浓度（IC）分别超过200 μg/mL和196.8 μg/mL。对小鼠的急性毒性、遗传毒性和诱变性评估显示无死亡、行为变化、生化改变、器官组织病理学损伤或对遗传物质的影响。SvO保护DNA免受MMS诱导的损伤。乙酸诱导的腹部扭体次数呈剂量依赖性减少，福尔马林试验第一和第二阶段的舔舐持续时间也减少。此外，SvO对角叉菜胶诱导的水肿具有抗水肿作用，在腹膜炎试验中中性粒细胞、白细胞、白细胞介素-1β和肿瘤坏死因子-α的迁移显著减少。
摄入SvO被证明是安全的，可保护DNA免受甲磺酸甲酯诱导的损伤，并具有抗伤害感受和抗炎作用。
