da Silva Santos Izabelly Bianca, de Freitas Rosa Simone Patricia, Pereira Wendel César eSilva, Dos Santos Bruno Souza, de Lima Natanael Teles Ramos, Filho José Maria Barbosa, de Oliveira Alisson Macário, Napoleão Thiago Henrique, Dos Santos Correia Maria Tereza, Costa Wêndeo Kennedy, da Silva Marcia Vanusa
Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, Pernambuco, 50670-901, Brazil.
Hospital Universitário Onofre Lopes-HUOL - Ebserh/UFRN, Natal, Rio Grande do Norte, 59012-300, Brazil.
Inflammopharmacology. 2025 Sep 26. doi: 10.1007/s10787-025-01972-y.
Myrciaria floribunda (H. West ex Willd.) O. Berg, a native species of the Myrtaceae family, is traditionally used in folk medicine and is known for its anticholinesterase, antioxidant, and antitumor properties. Despite this, its analgesic and anti-inflammatory effects remain underexplored. This study aimed to investigate the in vitro antioxidant and in vivo antinociceptive and anti-inflammatory activities of the hydroethanolic extract from M. floribunda leaves (MfHE). MfHE was prepared and analyzed by HPLC-DAD-MS, revealing 25 compounds, mainly tannins and flavonoids. Quantification showed 403.09 mg GAE/g of total phenolics and 2.94 mg QE/g of flavonoids. Antioxidant potential was evaluated using the DPPH assay (IC₅₀ = 63.24 μg/mL) and total antioxidant capacity (TAC 37.84%). Antinociceptive activity was assessed through acetic acid-induced writhing, formalin, and tail flick tests. MfHE (25, 50, and 100 mg/kg, p.o.) significantly reduced writhes by 35.30%, 47.06%, and 66.47%, respectively, inhibited both phases of the formalin test, and increased latency in the tail flick test by 100% at 100 mg/kg. Anti-inflammatory effects were evaluated in carrageenan-induced paw edema and peritonitis models. MfHE reduced paw edema at all doses and lowered total leukocytes (34.57%, 49.39%, 67.91%), neutrophils (28.31%, 52.84%, 60.33%), and pro-inflammatory cytokines IL-1β (52.46%, 66.84%, 75.85%) and TNF-α (44.75%, 69.05%, 87.26%) in peritonitis test. These findings suggest that MfHE, rich in polyphenols, has promising antioxidant, antinociceptive, and anti-inflammatory properties.
多花嘉宝果（Myrciaria floribunda (H. West ex Willd.) O. Berg）是桃金娘科的一种本土植物，传统上用于民间医学，以其抗胆碱酯酶、抗氧化和抗肿瘤特性而闻名。尽管如此，其镇痛和抗炎作用仍未得到充分研究。本研究旨在探讨多花嘉宝果树叶水乙醇提取物（MfHE）的体外抗氧化活性以及体内抗伤害感受和抗炎活性。制备了MfHE并通过HPLC-DAD-MS进行分析，鉴定出25种化合物，主要为单宁和黄酮类化合物。定量分析显示，总酚含量为403.09 mg GAE/g，黄酮类化合物含量为2.94 mg QE/g。使用DPPH法（IC₅₀ = 63.24 μg/mL）和总抗氧化能力（TAC 37.84%）评估抗氧化潜力。通过醋酸诱导扭体试验、福尔马林试验和甩尾试验评估抗伤害感受活性。MfHE（25、50和100 mg/kg，口服）分别使扭体次数显著减少35.30%、47.06%和66.47%，抑制福尔马林试验的两个阶段，在100 mg/kg时使甩尾试验的潜伏期延长100%。在角叉菜胶诱导的爪肿胀和腹膜炎模型中评估抗炎作用。MfHE在所有剂量下均能减轻爪肿胀，并降低腹膜炎试验中的总白细胞（34.57%、49.39%、67.91%）、中性粒细胞（28.31%、52.84%、60.33%）以及促炎细胞因子IL-1β（52.46%、66.84%、75.85%）和TNF-α（44.75%、69.05%、87.26%）水平。这些结果表明，富含多酚的MfHE具有良好的抗氧化、抗伤害感受和抗炎特性。
