Doyle Michelle R, Campo Paola, Dirik Selen, Balaguer Maria G, Martinez Angelica R, Kallupi Marsida, Palmer Abraham A, de Guglielmo Giordano
Department of Psychiatry, University of California San Diego, School of Medicine, La Jolla, California 92093.
Institute for Genomic Medicine, University of California San Diego, School of Medicine, La Jolla, California 92093.
eNeuro. 2025 Sep 12;12(9). doi: 10.1523/ENEURO.0207-25.2025. Print 2025 Sep.
Alcohol use disorder (AUD) imposes a significant global health burden, yet effective treatments remain limited. There are no well-characterized, AUD-relevant, rodent biological sample repositories to support research in this area. To address this gap, we established the Alcohol Biobank, a comprehensive resource containing thousands of samples from over 700 (half males, half females) genetically diverse heterogeneous stock (HS) rats. Modeled after two successful cocaine and oxycodone biobanks, this repository uses the chronic intermittent ethanol vapor exposure (CIE) model, paired with oral self-administration, to characterize AUD-like behaviors, including ethanol consumption, preference, motivation, and withdrawal symptoms such as allodynia and anxiety-like behavior. Longitudinal samples (blood, urine, and feces) are collected before, during, and after ethanol exposure, while tissue samples (brain, heart, kidneys, liver, cecum, reproductive organs, adrenal glands, blood) are obtained at intoxication, acute withdrawal, protracted abstinence, or from naive controls. Samples are preserved via snap-freezing or paraformaldehyde fixation to support diverse applications, including genomics, transcriptomics, proteomics, and neuroanatomy. Samples are freely available to nonprofit organizations at www.alcoholbiobank.org Genetic and behavioral data about these rats are deposited in public repositories. The Alcohol Biobank facilitates collaborative research to uncover biomarkers and develop novel therapies for AUD, addressing a critical need in addiction science.
酒精使用障碍（AUD）给全球健康带来了沉重负担，但有效的治疗方法仍然有限。目前尚无特征明确、与AUD相关的啮齿动物生物样本库来支持该领域的研究。为了填补这一空白，我们建立了酒精生物样本库，这是一个综合性资源库，包含来自700多只（雌雄各半）基因多样化的异质种群（HS）大鼠的数千份样本。该样本库以两个成功的可卡因和羟考酮生物样本库为模型，采用慢性间歇性乙醇蒸汽暴露（CIE）模型，并结合口服自我给药，来表征类似AUD的行为，包括乙醇消耗、偏好、动机以及诸如痛觉过敏和焦虑样行为等戒断症状。在乙醇暴露前、暴露期间和暴露后收集纵向样本（血液、尿液和粪便），而组织样本（脑、心脏、肾脏、肝脏、盲肠、生殖器官、肾上腺、血液）则在中毒、急性戒断、长期禁欲时获取，或从未接触过乙醇的对照组获取。样本通过速冻或多聚甲醛固定保存，以支持包括基因组学、转录组学、蛋白质组学和神经解剖学在内的多种应用。非营利组织可在www.alcoholbiobank.org免费获取这些样本。有关这些大鼠的遗传和行为数据已存入公共数据库。酒精生物样本库促进了合作研究，以发现生物标志物并开发针对AUD的新疗法，满足了成瘾科学领域的一项关键需求。
