Čutović Natalija, Batinić Petar, Marković Tatjana, Petrović Jovana, Obradović Milena, Bugarski Branko, Jovanović Aleksandra A
Institute for Medicinal Plants Research "Dr. Josif Pančić", Tadeuša Košćuška 1, 11000 Belgrade, Serbia.
Department of Plant Physiology, Institute for Biological Research "Siniša Stanković"-National Institute of Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, Serbia.
Foods. 2025 Aug 30;14(17):3064. doi: 10.3390/foods14173064.
The aim of this study was to evaluate liposomal particles as a potential delivery system for natamycin, a widely known antimicrobial agent used in the food industry. The goal was to prolong its diffusion into the surrounding medium. Natamycin-loaded liposomes were prepared using two methods (proliposome and thin-film) and two different phospholipid mixtures. The characterization of natamycin-loaded liposomes was performed in terms of their chemical composition (FT-IR analysis), encapsulation efficiency (EE), and antimicrobial potential against spoilage and pathogenic microorganisms that can be found in milk and milk products. During the 60-day storage period, their size, polydispersity index (PDI), and zeta potential were measured. The in vitro release kinetics of natamycin from liposomes were also assessed, and the results showed a significantly lower release rate of the drug when it was encapsulated. EE showed a high level of natamycin encapsulation (>80%), which was confirmed with FT-IR analysis. The stability study indicated that these systems were stable over a 60-day storage period, as the zeta potential of all formulations was ~-25 mV. Satisfactory antimicrobial performance of the developed liposomes against , , , , and (MIC values from 0.00625 to 4 mg/mL) indicates that loading of natamycin into liposomal carriers was an adequate method for their encapsulation and delivery in the milk industry.
本研究的目的是评估脂质体颗粒作为纳他霉素潜在递送系统的可能性，纳他霉素是食品工业中广泛使用的一种抗菌剂。目标是延长其在周围介质中的扩散时间。采用两种方法（前体脂质体法和薄膜法）和两种不同的磷脂混合物制备了载纳他霉素脂质体。对载纳他霉素脂质体进行了化学组成（傅里叶变换红外光谱分析）、包封率（EE）以及对牛奶和奶制品中可能存在的腐败微生物和致病微生物的抗菌潜力等方面的表征。在60天的储存期内，测量了它们的大小、多分散指数（PDI）和zeta电位。还评估了纳他霉素从脂质体中的体外释放动力学，结果表明药物被包封时释放速率显著降低。EE显示纳他霉素的包封率很高（>80%），这通过傅里叶变换红外光谱分析得到了证实。稳定性研究表明，这些体系在60天的储存期内是稳定的，因为所有制剂的zeta电位约为-25 mV。所制备的脂质体对[此处原文缺失具体微生物名称]具有令人满意的抗菌性能（最低抑菌浓度值为0.00625至4 mg/mL），这表明将纳他霉素负载到脂质体载体中是在牛奶工业中对其进行包封和递送的一种合适方法。
