蜜蜂益生菌菌株A3iob的基因组特征分析

Genomic Characterization of the Honeybee-Probiotic Strain A3iob.

作者信息

Elean Mariano, Arroyo Guerra Alejandro, Albarracin Leonardo, Nishiyama Keita, Kitazawa Haruki, Audisio M Carina, Villena Julio

机构信息

Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman CP4000, Argentina.

Instituto de Investigaciones para la Industria Química (INIQUI), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Salta, Salta CP4400, Argentina.

出版信息

Animals (Basel). 2025 Sep 5;15(17):2606. doi: 10.3390/ani15172606.

DOI:10.3390/ani15172606

PMID:40941401

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12427466/

Abstract

BACKGROUND

Previous studies have demonstrated the beneficial effects of A3iob on honeybee () colonies' health and honey production. The present work aimed to assess the genomic characteristics of the A3iob strain to understand its ability to improve bees' health.

METHODS

The comparative genomic analysis was performed with the A3iob genome and the genomes of probiotic strains of human, porcine, and chicken origin, as well as bacteria isolated from the bees' gut. The analysis included the examination of metabolic genes and functional genes related to adhesion, the production of bioactive compounds, the modulation of the host's immune system, and antimicrobial substances. Genes associated with antimicrobial resistance and virulence were also analyzed.

RESULTS

In silico studies revealed that A3iob possesses genes for glycosyltransferases (GTs) from the families GT2 and GT4, like and , and glycosylhydrolases (GH) from the families GH1, GH2, GH13, GH36, GH65, and GH177, similar to , , and bifidobacteria isolated from the bee intestine. The A3iob strain also has a unique genetic profile with a high number of secretion system genes and adhesion genes, including the ones coding for the SecA2/Y2 system, the mucus-binding proteins MucBP1, MucBP2, and MucBP3, and a pilus cluster (pilA, SpaA, SpaB, and sorteaseA) that has only been described in five strains of the species and in the intestinal bee-derived strain EDD2, which could be involved in the successful colonization of the A3iob strain in the bee gastrointestinal tract. Additionally, A3iob showed the presence of exopolysaccharide biosynthesis clusters described in the probiotic UCC118. Genes related to oxidative stress response (thioredoxin and NrdH-redoxin systems) and the bacteriocin genes abp118A and abp118B were found in the A3iob genome. A3iob does not harbor virulence or antibiotic resistance genes.

CONCLUSIONS

The genomic characterization of A3iob performed in this work provides some clues about the genetic mechanisms underlying its probiotic properties, paving the way for future research aimed at improving bees' health and productivity in the face of environmental challenges.

摘要

背景

先前的研究已证明A3iob对蜜蜂蜂群健康和蜂蜜产量具有有益影响。目前的工作旨在评估A3iob菌株的基因组特征，以了解其改善蜜蜂健康的能力。

方法

对A3iob基因组与源自人类、猪和鸡的益生菌菌株以及从蜜蜂肠道分离出的细菌的基因组进行了比较基因组分析。分析包括对与粘附、生物活性化合物产生、宿主免疫系统调节和抗菌物质相关的代谢基因和功能基因的检查。还分析了与抗菌抗性和毒力相关的基因。

结果

计算机模拟研究表明，A3iob拥有来自GT2和GT4家族的糖基转移酶（GTs）基因，如[未提及具体菌株名]和[未提及具体菌株名]，以及来自GH1、GH2、GH13、GH36、GH65和GH177家族的糖基水解酶（GH），类似于[未提及具体菌株名]、[未提及具体菌株名]以及从蜜蜂肠道分离出的双歧杆菌。A3iob菌株还具有独特的遗传特征，拥有大量分泌系统基因和粘附基因，包括编码SecA2/Y2系统、粘液结合蛋白MucBP1、MucBP2和MucBP3的基因，以及一个菌毛簇（pilA、SpaA、SpaB和分选酶A），这种菌毛簇仅在[未提及具体物种名]的五个菌株和蜜蜂肠道来源的菌株EDD2中被描述过，这可能与A3iob菌株在蜜蜂胃肠道中的成功定殖有关。此外，A3iob显示存在益生菌[未提及具体菌株名]UCC118中描述的胞外多糖生物合成簇。在A3iob基因组中发现了与氧化应激反应相关的基因（硫氧还蛋白和NrdH-氧化还原蛋白系统）以及细菌素基因abp118A和abp118B。A3iob不含有毒力或抗生素抗性基因。