Zhang Feng, Duan Yundan, Shang Qingmao
State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Plants (Basel). 2025 Aug 27;14(17):2656. doi: 10.3390/plants14172656.
Grafting is a common agricultural technique widely used in horticulture plants to improve stress tolerance, increase yield and improve quality. However, the biochemical processes underlying graft healing, especially the temporal dynamics and specific roles of polyphenolic metabolites, remain largely unknown. Polyphenols, widely present in plants, possess many biological activities, including antioxidant, antibacterial and anti-inflammatory properties. Temporal-resolution metabolomics, a powerful tool for studying metabolite dynamics over time, offers insights into the metabolic changes during this critical phase. To better understand this process, internodes above and below the graft site were harvested from 12 time points after grafting in both non-grafted and grafted peppers for polyphenolic metabolism analyses. In this study, we describe the accumulation pattern of 432 polyphenol metabolites. By comparing with the control group, nine polyphenolics were identified that responded to graft healing. The high temporal-resolution metabolomics reported here provides a basic resource for future functional study to unravel the genetic control of pepper graft development.
嫁接是一种常见的农业技术，广泛应用于园艺植物，以提高胁迫耐受性、增加产量和改善品质。然而，嫁接愈合背后的生化过程，尤其是多酚类代谢物的时间动态和具体作用，在很大程度上仍不清楚。多酚广泛存在于植物中，具有许多生物活性，包括抗氧化、抗菌和抗炎特性。时间分辨代谢组学是一种研究代谢物随时间动态变化的强大工具，它为这一关键阶段的代谢变化提供了见解。为了更好地理解这一过程，在嫁接后的12个时间点，从未嫁接和嫁接辣椒的嫁接部位上方和下方采集节间，进行多酚代谢分析。在本研究中，我们描述了432种多酚代谢物的积累模式。通过与对照组比较，鉴定出9种对嫁接愈合有反应的多酚。本文报道的高时间分辨率代谢组学为未来揭示辣椒嫁接发育的遗传控制的功能研究提供了基础资源。
