Aniya Y, Sakanashi M, Noguchi K, Matsusaki K
Jpn J Pharmacol. 1985 Dec;39(4):437-41. doi: 10.1254/jjp.39.437.
The biological activity of a Habu (Trimeresurus flavoiridis) venom fraction with drug-metabolizing enzyme inhibitory action was studied. The venom fraction, which was isolated through Sephadex G-100 gel filtration and cation exchange chromatography on Amberlite CG50, caused an increase of vascular permeability and hemorrhage, but these actions were lost after heating at 70 degrees C for 5 min. The fraction showed anticoagulant activity on citrated blood, and this activity remained after heating of the venom. Guinea pig ileum was contracted by treatment with nonheated or heated venom fraction, and these contractions were inhibited with atropine and potentiated with physostigmine. These results suggest that the drug-metabolizing enzyme inhibitor isolated from Habu venom involves the heat stable component with anticoagulant activity and smooth muscle contractile action.
对具有药物代谢酶抑制作用的哈布蛇（竹叶青蛇）毒液组分的生物活性进行了研究。通过Sephadex G - 100凝胶过滤和Amberlite CG50阳离子交换色谱法分离得到的毒液组分，可引起血管通透性增加和出血，但在70℃加热5分钟后这些作用消失。该组分对枸橼酸化血液具有抗凝活性，且加热毒液后该活性仍然存在。用未加热或加热的毒液组分处理豚鼠回肠会引起收缩，这些收缩可被阿托品抑制，被毒扁豆碱增强。这些结果表明，从哈布蛇毒液中分离出的药物代谢酶抑制剂涉及具有抗凝活性和平滑肌收缩作用的热稳定成分。
