Üzer Fatime Betül, Helvacı Nazlı, Elmastaş Mahfuz
Department of Phytotherapy, University of Health Sciences, Selimiye Complex, Selimiye Neighborhood, Atolyeler Street No: 4, Uskudar, 34668 Istanbul, Turkey.
Molecules. 2025 Aug 22;30(17):3450. doi: 10.3390/molecules30173450.
This study aimed to evaluate the inhibitory potential of phenolic-rich extracts from selected medicinal plants belonging to the and families against the furin protease enzyme, a key target in viral and oncogenic pathways. Extracts of , , , , , and were prepared using hexane, chloroform, and ethyl acetate. Phenolic compounds were quantified using High-Performance Liquid Chromatography (HPLC). Furin inhibition was assessed spectrophotometrically and analyzed statistically with multivariate approaches. The chloroform extract of exhibited 97.44 ± 0.12% inhibition, while the ethyl acetate extract of showed 97.44 ± 0.08% inhibition. Epicatechin and rutin displayed significant synergistic effects, while naringenin negatively affected inhibition ( < 0.05). Solvent polarity significantly influenced phenolic diversity and biological activity, with ternary combinations showing 33% higher inhibition than single compounds. These findings highlight phenolic blends as promising natural furin inhibitors, with chloroform being optimal for broad-spectrum extraction.
本研究旨在评估来自伞形科和唇形科某些药用植物的富含酚类提取物对弗林蛋白酶的抑制潜力，弗林蛋白酶是病毒和致癌途径中的关键靶点。使用己烷、氯仿和乙酸乙酯制备了当归、迷迭香、薰衣草、薄荷、牛至和百里香的提取物。使用高效液相色谱法（HPLC）对酚类化合物进行定量。通过分光光度法评估弗林蛋白酶抑制作用，并采用多变量方法进行统计分析。当归的氯仿提取物表现出97.44±0.12%的抑制率，而牛至的乙酸乙酯提取物显示出97.44±0.08%的抑制率。表儿茶素和芦丁表现出显著的协同作用，而柚皮苷对抑制作用有负面影响（P<0.05）。溶剂极性显著影响酚类多样性和生物活性，三元组合的抑制作用比单一化合物高33%。这些发现突出了酚类混合物作为有前景的天然弗林蛋白酶抑制剂的潜力，氯仿是广谱提取的最佳溶剂。
