Zhang Xu, An Siyu, Zhou Lanying, Chen Chen, Yang Xue
Jilin Province Product Quality Supervision and Inspection Institute, Changchun 130103, China.
Molecules. 2025 Aug 29;30(17):3536. doi: 10.3390/molecules30173536.
In this study, a novel polysaccharide (PSH) with potent anti-gout activity was extracted and separated from (). The structural characteristics of PSH were elucidated using analytical techniques. HPLC analysis revealed that PSH was a heteropolysaccharide with a molecular weight of 5.25 × 10 Da. FT-IR, NMR, and GC-MS collectively demonstrated that PSH was a pyranose with both α and β configurations, primarily composed of Glcp-(1→, →4)-Glcp-(2→, →3)-Galp-(1→, and Araf-(1→ linkages. The cell viability confirmed the non-toxicity of PSH. CAT and SOD showed that compared with the model group, PSH significantly offset the oxidative damage induced by MSU ( < 0.01). The results from ROS and MDA mutually corroborated the antioxidant capacity of PSH. Furthermore, PSH effectively suppressed MSU-triggered inflammatory responses. The antioxidant and anti-inflammatory experiments provided evidence for the anti-gout efficacy of PSH. Collectively, these findings support the potential development of PSH as an anti-gout active substance.
在本研究中，从（）中提取并分离出一种具有强大抗痛风活性的新型多糖（PSH）。采用分析技术阐明了PSH的结构特征。高效液相色谱分析表明，PSH是一种分子量为5.25×10 Da的杂多糖。傅里叶变换红外光谱、核磁共振和气相色谱 - 质谱联用分析共同表明，PSH是一种同时具有α和β构型的吡喃糖，主要由Glcp-(1→, →4)-Glcp-(2→, →3)-Galp-(1→和Araf-(1→连接键组成。细胞活力证实了PSH的无毒性。过氧化氢酶（CAT）和超氧化物歧化酶（SOD）表明，与模型组相比，PSH显著抵消了单钠尿酸盐（MSU）诱导的氧化损伤（P < 0.01）。活性氧（ROS）和丙二醛（MDA）的结果相互印证了PSH的抗氧化能力。此外，PSH有效抑制了MSU引发的炎症反应。抗氧化和抗炎实验为PSH的抗痛风功效提供了证据。总体而言，这些发现支持了PSH作为抗痛风活性物质的潜在开发。
