Aalizadeh Majid, Yang Shuo, Guntur Suchithra, Potluri Vaishnavi, Kulkarni Girish, Fan Xudong
Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, MI 48109, USA.
Sensors (Basel). 2025 Aug 24;25(17):5262. doi: 10.3390/s25175262.
In this work, a miniaturized, automated, and cost-effective ELISA device is designed and implemented, without the utilization of conventional techniques such as pipetting or microfluidic valve technologies. The device has dimensions of 24 cm × 19 cm × 14 cm and weighs <3 kg. The total hardware cost of the device is estimated to be approximately $1200, which can be further reduced through optimization during scale-up production. Three-dimensional printed disposable parts, including the reagent reservoir disk and the microfluidic connector, have also been developed. IL-6 is used as a model system to demonstrate how the device provides an ELISA measurement. The cost per test is estimated to be <$10. The compactness, automated operation, along with the cost-effectiveness of this ELISA device, makes it suitable for point-of-care applications in resource-limited regions.
在这项工作中，设计并实现了一种小型化、自动化且具有成本效益的酶联免疫吸附测定（ELISA）设备，无需使用诸如移液或微流控阀技术等传统技术。该设备尺寸为24厘米×19厘米×14厘米，重量小于3千克。该设备的硬件总成本估计约为1200美元，在扩大规模生产过程中通过优化可进一步降低成本。还开发了三维打印的一次性部件，包括试剂储存盘和微流控连接器。白细胞介素-6（IL-6）用作模型系统，以演示该设备如何进行ELISA测量。每次测试的成本估计低于10美元。这种ELISA设备的紧凑性、自动化操作以及成本效益，使其适用于资源有限地区的即时检测应用。
