Wang Ping, Song Yi, Jiang Haixin, Qi Chenyuan, Zhang Xubo, Wang Disheng, Li Luqi, Zhang Qiang
Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling 712100, China.
Life Science Research Core Services, Northwest A&F University, Yangling 712100, China.
Int J Mol Sci. 2025 Aug 22;26(17):8112. doi: 10.3390/ijms26178112.
Peach () leaves, usually discarded in traditional Chinese medicine, were explored as a source of laxative agents. Using zebrafish larvae for bioactivity-guided fractionation, we isolated a single active flavanone that was identified by NMR and HR-MS as Sakuranetin. In vivo assays demonstrated that Sakuranetin (10-25 µM) accelerated intestinal transit in a dose-dependent fashion; at 25 µM, 64.8% of the fluorescent intestinal content was expelled. Untargeted LC-MS metabolomic analysis revealed significant perturbations in serine biosynthesis and N-glycan precursor biosynthesis, suggesting energetic rewiring of enterocytes. RNA-Seq analysis highlighted as the most responsive gene, and molecular docking predicted a stable Sakuranetin-Gnat1 complex with a binding free energy of -8.7 kcal/mol. Concurrent down-regulation of transcripts indicated suppression of inflammatory signaling that often accompanies constipation. Our findings identified Sakuranetin as a potent promoter of gut motility and position the otherwise wasted peach leaves as an untapped botanical resource for developing anti-constipation therapeutics.
通常在传统中药中被丢弃的桃（）叶，被作为泻药来源进行了探索。利用斑马鱼幼虫进行生物活性导向的分级分离，我们分离出一种单一的活性黄烷酮，通过核磁共振（NMR）和高分辨质谱（HR-MS）鉴定为樱花素。体内试验表明，樱花素（10 - 25 μM）以剂量依赖的方式加速肠道运输；在25 μM时，64.8%的荧光肠内容物被排出。非靶向液相色谱-质谱代谢组学分析显示丝氨酸生物合成和N-聚糖前体生物合成存在显著扰动，提示肠细胞的能量重新布线。RNA测序分析突出显示 为最敏感的基因，分子对接预测樱花素与Gnat1形成稳定复合物，结合自由能为 - 8.7千卡/摩尔。 转录本的同时下调表明炎症信号传导受到抑制，而炎症信号传导常伴随便秘。我们的研究结果确定樱花素是肠道运动的有效促进剂，并将原本被浪费的桃叶定位为开发抗便秘疗法的未开发植物资源。
