Cho Won-Kyung, Ma Jin Yeul
Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, 70 Cheomdan-ro, Dong-gu, Daegu 41062, Republic of Korea.
Int J Mol Sci. 2025 Aug 22;26(17):8158. doi: 10.3390/ijms26178158.
Deoxyshikonin (DS) is a derivative of shikonin, the main compound present in , the root of Siebold and Zucc. In this study, we investigated the antiviral effects of DS using Influenza A/PR8/34, which expresses green fluorescent protein (GFP) as well as wild-type PR8/34 H1N1 Influenza A virus (IAV). Fluorescence microscopy and flow cytometry results showed that DS from 1.25 to 5 µM significantly and dose-dependently inhibited PR8-GFP IAV infection. A plaque assay confirmed the inhibitory effect of DS against H1N1 IAV infection. Consistently, immunofluorescence results showed that DS suppresses IAV protein expression. Time-of-drug-addition and hemagglutination inhibition assays revealed that DS exhibits anti-influenza virus efficacy by blocking the viral attachment and penetration into the cells and has a direct virus-eradication effect in the early stages of infection. However, DS did not repress neuraminidase activity. Our findings suggest that DS could be used not only to protect against the early stages of IAV infection, but also to treat influenza virus infections in combination with NA inhibitors.
去氧紫草素（DS）是紫草素的衍生物，紫草素是紫草根中的主要化合物。在本研究中，我们使用表达绿色荧光蛋白（GFP）的甲型/PR8/34流感病毒以及野生型PR8/34 H1N1甲型流感病毒（IAV）研究了DS的抗病毒作用。荧光显微镜和流式细胞术结果表明，浓度为1.25至5 μM的DS能显著且呈剂量依赖性地抑制PR8-GFP IAV感染。蚀斑试验证实了DS对H1N1 IAV感染的抑制作用。同样，免疫荧光结果表明DS可抑制IAV蛋白表达。药物添加时间和血凝抑制试验表明，DS通过阻断病毒与细胞的附着和穿透发挥抗流感病毒作用，并在感染早期具有直接的病毒清除作用。然而，DS并未抑制神经氨酸酶活性。我们的研究结果表明，DS不仅可用于预防IAV感染的早期阶段，还可与神经氨酸酶抑制剂联合用于治疗流感病毒感染。
