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山小橘黄酮抗流感病毒的作用。

Antiviral Effect of Amentoflavone Against Influenza Viruses.

机构信息

Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM), 70 Cheomdan-ro, Dong-gu, Daegu 41062, Republic of Korea.

Department of Medical Biotechnology, Yeungnam University, Gyeongsan 38541, Republic of Korea.

出版信息

Int J Mol Sci. 2024 Nov 19;25(22):12426. doi: 10.3390/ijms252212426.

DOI:10.3390/ijms252212426
PMID:39596490
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11595079/
Abstract

Amentoflavone (AF) is a biflavonoid compound found in many plants. In this study, we first demonstrate that AF has a potent antiviral effect against the influenza virus via the inhibition of viral attachment and virucidal effects. The anti-influenza-viral effect of AF was evaluated using green fluorescent protein-tagged Influenza A virus (IAV) with fluorescent microscopy and flow cytometry analysis. AF decreased the GFP expression by viral infection, dose-dependently. Fifty micromoles of AF suppressed the GFP expression by virus infection of up to 70% of untreated infected control cells. Consistently, immunofluorescence results showed the inhibitory effect of AF on viral protein expression. Time-of-addition and hemagglutination assays revealed that AF inhibits viral binding to cells by interfering with the hemagglutinin (HA) of IAV. Furthermore, AF has a virucidal effect and blocks cytopathic effects caused by the Influenza B virus and H3N2 IAV. Additionally, AF represses the neuraminidase (NA) activity of IAV. In silico analysis confirmed the potential interaction of AF with both HA and NA. Our findings indicate that AF has antiviral effects by modulating HA and NA during the attachment and release stages of influenza viral infection.

摘要

穗花杉双黄酮（AF）是一种存在于多种植物中的双黄酮类化合物。在这项研究中，我们首先通过抑制病毒附着和病毒杀伤作用证明 AF 对流感病毒具有很强的抗病毒作用。通过荧光显微镜和流式细胞术分析，用绿色荧光蛋白标记的甲型流感病毒（IAV）评估 AF 的抗流感病毒作用。AF 可降低病毒感染的 GFP 表达，呈剂量依赖性。50μM 的 AF 可抑制病毒感染导致的 GFP 表达，抑制率高达未处理感染对照细胞的 70%。免疫荧光结果也显示了 AF 对病毒蛋白表达的抑制作用。时效加入和血凝试验表明，AF 通过干扰 IAV 的血凝素（HA）抑制病毒与细胞的结合。此外，AF 具有病毒杀伤作用，并阻断乙型流感病毒和 H3N2 IAV 引起的细胞病变效应。此外，AF 还可抑制 IAV 的神经氨酸酶（NA）活性。计算机模拟分析证实了 AF 与 HA 和 NA 的潜在相互作用。我们的研究结果表明，AF 通过在流感病毒感染的附着和释放阶段调节 HA 和 NA 发挥抗病毒作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c239/11595079/9c11bc8a88ff/ijms-25-12426-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c239/11595079/73df1ecb744f/ijms-25-12426-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c239/11595079/c2b2132bac53/ijms-25-12426-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c239/11595079/693ebc06907e/ijms-25-12426-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c239/11595079/097cfbb2813b/ijms-25-12426-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c239/11595079/6ff26ccc0b66/ijms-25-12426-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c239/11595079/8fe324185cdc/ijms-25-12426-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c239/11595079/9c11bc8a88ff/ijms-25-12426-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c239/11595079/73df1ecb744f/ijms-25-12426-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c239/11595079/c2b2132bac53/ijms-25-12426-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c239/11595079/693ebc06907e/ijms-25-12426-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c239/11595079/097cfbb2813b/ijms-25-12426-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c239/11595079/6ff26ccc0b66/ijms-25-12426-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c239/11595079/8fe324185cdc/ijms-25-12426-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c239/11595079/9c11bc8a88ff/ijms-25-12426-g007.jpg

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