Pertusa Clara, Carrasco-García Álvaro, Aliaga Rosa, Suay Loreto, Alonso-Iglesias Eulalia, Cano Antonio, Tarín Juan J, García-Pérez Miguel Ángel
Research Unit, INCLIVA Biomedical Research Institute, 46010 Valencia, Spain.
Department of Internal Medicine, Erasmus MC, 3015 CE Rotterdam, The Netherlands.
Int J Mol Sci. 2025 Sep 1;26(17):8484. doi: 10.3390/ijms26178484.
Despite the rising incidence of osteoporosis (the most common bone disorder) as life expectancy increases worldwide, the genetic and metabolic factors contributing to this multifactorial disease are still poorly understood. This study investigated the role of arginine metabolism in bone formation and its potential for preventing bone loss in postmenopausal osteoporosis. The osteogenic effects of arginine were evaluated in vitro by determining calcium mineral deposition and the expression of marker genes in the human osteoblastic cell line Saos-2. In vivo analyses were conducted in ovariectomized mice treated with arginine, focusing on femoral bone microarchitecture, marker gene expression and serum metabolite profiles. Arginine treatment enhanced calcium deposition and osteoblastic differentiation in vitro. In contrast, however, this treatment had a deleterious effect in vivo, exacerbating trabecular bone loss. These results are particularly relevant given the wide availability of arginine as a dietary supplement, and our findings underscore the necessity of verifying the safety of nutritional supplements in different populations and in the presence of disease.
尽管随着全球预期寿命的增加，骨质疏松症（最常见的骨骼疾病）的发病率不断上升，但导致这种多因素疾病的遗传和代谢因素仍未得到充分了解。本研究调查了精氨酸代谢在骨形成中的作用及其预防绝经后骨质疏松症骨质流失的潜力。通过测定人成骨细胞系Saos-2中的钙矿物质沉积和标记基因的表达，在体外评估了精氨酸的成骨作用。在用精氨酸治疗的去卵巢小鼠中进行了体内分析，重点关注股骨骨微结构、标记基因表达和血清代谢物谱。精氨酸处理增强了体外钙沉积和成骨细胞分化。然而，相比之下，这种处理在体内具有有害作用，加剧了小梁骨丢失。鉴于精氨酸作为膳食补充剂广泛可得，这些结果尤为重要，我们的研究结果强调了在不同人群和疾病存在的情况下验证营养补充剂安全性的必要性。
