Shakeri Abroudi Ali, Mashhouri Moghaddam Mahtab, Hashemi Karoii Danial, Djamali Melika, Azizi Hossein, Skutella Thomas
Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran 1477893855, Iran.
Department of Pharmacy, Yeditepe University, Istanbul 34755, Turkey.
Int J Mol Sci. 2025 Sep 3;26(17):8575. doi: 10.3390/ijms26178575.
A complex and gradual process, the epithelial-mesenchymal transition (EMT) occurs both during embryonic development and tumor progression. Cells undergo a transition from an epithelial to a mesenchymal state throughout this process. More and more evidence points to EMT as a cause of increased metastatic spread of prostate cancer (PCa), along with stemness enhancement and therapy resistance. Here, we used bioinformatic methods to analyze gene expression microarray data, single-cell RNA sequencing, oncogenes, and tumor suppressor genes (TSGs) in order to reconstruct the network of differentially expressed genes (DEGs) involved in the epithelial-mesenchymal transition with PCa. No prior study has documented this sort of analysis. We next validated our results using data from the Cancer Genome Atlas (TCGA), which included microarray and single-cell RNA sequencing. Potentially useful in PCa diagnosis and treatment are extracellular matrix in epithelial-mesenchymal transition genes, including , , , , , , and . In this study, we aimed to shed light on the molecular characteristics and pathways of DEGs in PCa, as well as to identify possible biomarkers that are important in the development and advancement of this cancer. These insights have important implications for understanding prostate cancer progression and for the development of therapeutic strategies targeting ECM-mediated pathways.
上皮-间质转化（EMT）是一个复杂且渐进的过程，在胚胎发育和肿瘤进展过程中均会发生。在此过程中，细胞经历从上皮状态到间质状态的转变。越来越多的证据表明，EMT是前列腺癌（PCa）转移扩散增加的原因，同时还伴随着干性增强和治疗抵抗。在这里，我们使用生物信息学方法分析基因表达微阵列数据、单细胞RNA测序、癌基因和肿瘤抑制基因（TSG），以重建与PCa上皮-间质转化相关的差异表达基因（DEG）网络。此前尚无研究记录过此类分析。接下来，我们使用来自癌症基因组图谱（TCGA）的数据验证了我们的结果，其中包括微阵列和单细胞RNA测序。上皮-间质转化基因中的细胞外基质，包括 、 、 、 、 、 和 ，可能在PCa诊断和治疗中发挥作用。在本研究中，我们旨在阐明PCa中DEG的分子特征和途径，以及识别在这种癌症的发生和发展中重要的潜在生物标志物。这些见解对于理解前列腺癌进展以及开发针对ECM介导途径的治疗策略具有重要意义。
