Somera Isabella K, Sosa Bryan, Cottrell Jessica A
Department of Biological Sciences, Seton Hall University, South Orange, NJ 07079, USA.
Int J Mol Sci. 2025 Sep 5;26(17):8682. doi: 10.3390/ijms26178682.
Osteoblastogenesis plays a critical role in bone repair. Insulin and insulin-mimetic compounds, such as vanadium (IV) oxide acetylacetonate (VAC), have been reported to enhance bone healing in various models. This study aimed to evaluate the effects of vanadium compounds, VAC and vanadium (IV) oxide sulfate (VOSO), on osteoblast proliferation and function. MC3T3-E1 pre-osteoblast cells were treated with insulin, ascorbic acid, and varying concentrations of VAC or VOSO, and samples were collected at multiple time points over 21 days. We assessed cell proliferation, functional markers, and gene and protein expression. Our findings demonstrate that both VAC and VOSO stimulate MC3T3-E1 proliferation, increase calcium and proteoglycan deposition, and enhance phosphorylation of Protein Kinase B (Akt) over time. Gene expression analysis revealed that VAC treatment upregulated RUNX2, BGLAP, and TWIST2 at Day 7 compared to controls, with sustained expression patterns observed at Day 10. These results align with existing literature, supporting that VAC and VOSO promote osteoblastogenesis and may serve as effective adjuvants to accelerate bone regeneration during fracture healing.
成骨细胞生成在骨修复中起着关键作用。胰岛素和胰岛素模拟化合物，如乙酰丙酮氧化钒（IV）（VAC），已报道在各种模型中可促进骨愈合。本研究旨在评估钒化合物VAC和硫酸氧化钒（IV）（VOSO）对成骨细胞增殖和功能的影响。用胰岛素、抗坏血酸和不同浓度的VAC或VOSO处理MC3T3-E1前成骨细胞，并在21天内的多个时间点收集样本。我们评估了细胞增殖、功能标志物以及基因和蛋白质表达。我们的研究结果表明，VAC和VOSO均能刺激MC3T3-E1增殖，增加钙和蛋白聚糖沉积，并随着时间的推移增强蛋白激酶B（Akt）的磷酸化。基因表达分析显示，与对照组相比，VAC处理在第7天上调了RUNX2﹑BGLAP和TWIST2，在第10天观察到持续的表达模式。这些结果与现有文献一致，支持VAC和VOSO促进成骨细胞生成，并可能作为有效的佐剂在骨折愈合过程中加速骨再生。
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