Sherstyukova Ekaterina, Semenova Julia, Kandrashina Snezhanna, Bogdanova Alina, Vinogradov Ilya, Inozemtsev Vladimir, Shvedov Mikhail, Grechko Alexander, Dokukin Maxim, Kuzovlev Artem, Klychnikova Elena, Bulanov Andrey, Kostin Alexander, Sergunova Viktoria
Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, Moscow 107031, Russia.
Moscow Department of Health, Sklifosovsky Research Institute for Emergency Medicine, Moscow 129090, Russia.
J Clin Med. 2025 Sep 5;14(17):6292. doi: 10.3390/jcm14176292.
: Leukoreduced low-titer group O whole blood (LTOWB) is a promising option for early transfusion in massive hemorrhages, by providing red blood cells (RBCs), plasma, and platelets in a single unit. This study aimed to assess the effects of pathogen reduction (PR) on the LTOWB quality and its suitability for emergency transfusions, including its post-thaw RBC usability. : Whole blood from 24 donors was processed and analyzed over seven days, including post-thaw assessments. Hematological and coagulation parameters, along with atomic force microscopy, were used to assess the RBCs' morphology, cytoskeletal integrity, and nanomechanical properties. : The PR reduced the leukocyte content while preserving platelet counts at the level of the control group. Although certain clotting factors, such as fibrinogen ( < 0.001) and factor VIII ( < 0.001), were diminished after the PR, thromboelastometry results remained within reference ranges, indicating a maintained hemostatic potential. Morphological changes in RBCs were observed, but the membrane elasticity remained stable throughout storage and after thawing, indicating preserved macromechanical properties essential for hemostasis. : These findings demonstrate that LTOWB treated with partial a leukoreduction and PR maintains functional and structural integrity, supporting its potential utility as a safe and effective transfusion product for managing acute blood loss.
白细胞滤除的低滴度O型全血（LTOWB）是大出血早期输血的一个有前景的选择，因为它能在一个单位中提供红细胞（RBC）、血浆和血小板。本研究旨在评估病原体灭活（PR）对LTOWB质量及其用于紧急输血的适用性的影响，包括解冻后红细胞的可用性。
对24名献血者的全血进行了为期七天的处理和分析，包括解冻后评估。使用血液学和凝血参数以及原子力显微镜来评估红细胞的形态、细胞骨架完整性和纳米力学性能。
PR降低了白细胞含量，同时使血小板计数维持在对照组水平。虽然PR后某些凝血因子，如纤维蛋白原（<0.001）和凝血因子VIII（<0.001）减少，但血栓弹力图结果仍在参考范围内，表明止血潜力得以维持。观察到红细胞有形态变化，但在整个储存过程和解冻后膜弹性保持稳定，表明维持了止血所需的宏观力学性能。
这些发现表明，经过部分白细胞滤除和PR处理的LTOWB保持了功能和结构完整性，支持其作为管理急性失血的安全有效输血产品的潜在用途。
