Li Guangjin, Zhang Zhanquan, Chen Yong, Xing Mengyang, Chen Tong, Li Boqiang, Tian Shiping
State Key Laboratory of Plant Diversity and Specialty Crops, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China.
China National Botanical Garden, Beijing, 100093, China.
Sci China Life Sci. 2025 Sep 11. doi: 10.1007/s11427-025-2986-6.
Light, as an important environmental factor, has a crucial influence on the life activities of fungi. Botrytis cinerea is a typical light-responsive filamentous fungus capable of coordinating its growth and development with ambient light signals. Here, we find that Bcmads1, a key transcription factor in the light signaling pathway, can regulate the accumulation of many metabolites in a light-dependent manner, and demonstrate that N-vanillylnonanamide plays an important role in Bcmads1-regulated photomorphogenesis. Then, we confirm that Bcmads1 can directly regulate the expression of BcAMT1, which further affects the photomorphogenesis of B. cinerea by catalyzing the reaction from vanillin to vanillylamine in the N-vanillylnonanamide synthesis pathway. We find a new pathway of light signal transduction in B. cinerea and elucidate the new mechanism of Bcmads1 regulating N-vanillylnonanamide synthesis involved in the photomorphogenesis of B. cinerea.
光作为一种重要的环境因素，对真菌的生命活动有着至关重要的影响。灰葡萄孢是一种典型的光响应丝状真菌，能够根据环境光信号协调其生长和发育。在此，我们发现光信号通路中的关键转录因子Bcmads1可以以光依赖的方式调节多种代谢物的积累，并证明N-香草基壬酰胺在Bcmads1调控的光形态建成中起重要作用。然后，我们证实Bcmads1可以直接调控BcAMT1的表达，BcAMT1通过催化N-香草基壬酰胺合成途径中从香草醛到香草胺的反应，进一步影响灰葡萄孢的光形态建成。我们发现了灰葡萄孢中光信号转导的一条新途径，并阐明了Bcmads1调控参与灰葡萄孢光形态建成的N-香草基壬酰胺合成的新机制。
