Cai Yunfei, Ying Jiali, Ye Youju, Wen Shuangshuang, Qian Renjuan
Zhejiang Institute of Subtropical Crops, Zhejiang Academy of Agricultural Sciences, Xueshan Road 334, Wenzhou, 325005, People's Republic of China.
Plant Cell Rep. 2025 Jun 29;44(7):160. doi: 10.1007/s00299-025-03559-x.
Blue light treatment can stimulate antioxidant enzyme activity and induce JA synthesis through CRY1a enhancing the resistance of Solanum lycopersicum to Botrytis cinerea. Light signals are prevalent in the environment and significantly influence the growth, development, plant resistance, and pathogenicity of phytopathogenic fungi. The agricultural application of spectral engineering through optimized red and blue light proportions has been used as a practical methodology for synergistically improving plant photobiology, nutritional metabolism, and environmental adaptation capabilities. However, its role in plant disease resistance has not been comprehensively explored. In our study, the pathogenicity analysis indicates that blue light significantly enhances the resistance of Solanum lycopersicum to Botrytis cinerea. Transcriptomic profiling revealed that blue light activates OPR3 and JAR1 expression, concomitant with elevated jasmonic acid biosynthesis and significantly enhanced activities of key antioxidant enzymes including peroxidase, catalase, and ascorbate peroxidase in S. lycopersicum. Furthermore, the mutation of the blue light receptor cryptochrome 1a (CRY1a) was found to enhance S. lycopersicum resistance to B. cinerea. For the pathogen B. cinerea, blue light was observed to induce DHN-melanin synthesis-related genes Bop2, Bcbrn2, Bcpks4, and Bcpks21 expression, and the development of its infection cushion was notably slower under blue light in B. cinerea. The above results indicate that blue light can control tomato gray mold by enhancing S. lycopersicum resistance and suppressing B. cinerea infection, which suggests that blue light may possess potential application value in disease management for facility-based S. lycopersicum cultivation. Our study reveals how blue light and light receptor CRY1a function in S. lycopersicum to defend necrotrophic fungal pathogens.
蓝光处理可以通过CRY1a刺激抗氧化酶活性并诱导茉莉酸（JA）合成，从而增强番茄对灰霉病的抗性。光信号在环境中普遍存在，并显著影响植物的生长、发育、抗性以及植物病原真菌的致病性。通过优化红光和蓝光比例进行光谱工程的农业应用已成为一种协同改善植物光生物学、营养代谢和环境适应能力的实用方法。然而，其在植物抗病性方面的作用尚未得到全面探索。在我们的研究中，致病性分析表明蓝光显著增强了番茄对灰霉病的抗性。转录组分析显示，蓝光激活了OPR3和JAR1的表达，同时伴随着番茄中茉莉酸生物合成的升高以及包括过氧化物酶、过氧化氢酶和抗坏血酸过氧化物酶在内的关键抗氧化酶活性的显著增强。此外，发现蓝光受体隐花色素1a（CRY1a）的突变增强了番茄对灰霉病的抗性。对于病原菌灰霉病，观察到蓝光诱导了与DHN-黑色素合成相关的基因Bop2、Bcbrn2、Bcpks4和Bcpks21的表达，并且在蓝光下灰霉病感染垫的发育明显减缓。上述结果表明，蓝光可以通过增强番茄抗性和抑制灰霉病感染来控制番茄灰霉病，这表明蓝光在设施番茄栽培的病害管理中可能具有潜在的应用价值。我们的研究揭示了蓝光和光受体CRY1a在番茄中抵御坏死营养型真菌病原体的作用机制。
