Chowdhury Mohiuddin Amirul Kabir, Lahiry Ankon, Rahi Md Lifat, Hossain Md Amzad, de Aguiar Gustavo, Alves Graziela, Aye Aung Tun, Dutta Rajib, Bonato Melina Aparecida, Tacon Albert G J
Maverick Innovation, Kaliakair, Gazipur, Bangladesh.
Fisheries and Marine Resource Technology, Khulna University, Khulna, Bangladesh.
Aquac Nutr. 2025 Sep 5;2025:7934851. doi: 10.1155/anu/7934851. eCollection 2025.
The effects of graded levels of hydrolyzed yeast (HY) supplementation (0.0, 0.5, 1.0, and 2.0 g/kg, i.e., Control, HY0.5, HY1.0, HY2.0, respectively) on growth performance, gut health, and immune responses of juvenile Nile tilapia () were assessed in this study. The experiment was conducted in a completely randomized design for 14 weeks, where the treatments were distributed in 16 300-L tanks with four replicates each. Despite no significant differences, the final body weight and weight gain were numerically higher in treatments containing HY (44.7 and 34.7 g, 43.5 and 33.5 g, and 45.5 and 35.5 g in HY0.5, HY1.0, and HY2.0, respectively). Feed efficiency (FE) was improved linearly ( < 0.05) with increasing dietary HY level (0.65, 0.70, and 0.72, respectively). Similarly, there was also a significant linear relationship between protein deposition (PD), as well as protein and energy retention efficiency (ERE), with the increasing dietary HY level. Among the blood parameters, only the hematocrit (HCT) value was significantly lower in HY1.0 and HY2.0 compared to the control and HY0.5 treatments. Gut histology showed significantly higher villi length in fish fed HY2.0 diets (795 ± 89.6 µm) compared to those fed the control diet (504 ± 80.7 µm). The average surface volume (SV) of the villi was also higher in tilapia fed HY0.5, HY1.0, and HY2.0 diets (0.025, 0.026, and 0.038 mm, respectively) compared to the control diet (0.021 mm). All four target genes were significantly upregulated in HY1.0 and HY2.0 treatments. The expression of the genes supporting growth and ATP production, insulin-like growth factor-1 (IGF-1) and glyceraldehyde-3-phosphate (G-3-P), respectively, was significantly improved, as well as the expression of the immune-related gene, hepcidin. The expression of ghrelin also showed a significant increase with increasing HY levels. It can be concluded that the HY supplementation improved feed utilization, gut health, nutrient absorption capacity, and immunity in Nile tilapia.
本研究评估了不同添加水平（0.0、0.5、1.0和2.0 g/kg，即分别为对照组、HY0.5、HY1.0、HY2.0）的水解酵母（HY）对尼罗罗非鱼幼鱼生长性能、肠道健康和免疫反应的影响。实验采用完全随机设计，为期14周，各处理分布于16个300升的水箱中，每个处理重复4次。尽管无显著差异，但添加HY的处理组最终体重和增重数值上更高（HY0.5、HY1.0和HY2.0组分别为44.7和34.7克、43.5和33.5克、45.5和35.5克）。随着饲料中HY水平的增加，饲料效率（FE）呈线性提高（P<0.05）（分别为0.65、0.70和0.72）。同样，蛋白质沉积（PD）以及蛋白质和能量保留效率（ERE）与饲料中HY水平的增加也存在显著的线性关系。在血液参数中，与对照组和HY0.5处理相比，仅HY1.0和HY2.0组的血细胞比容（HCT）值显著降低。肠道组织学显示，与饲喂对照饲料（504±80.7μm）的鱼相比，饲喂HY2.0饲料的鱼绒毛长度显著更长（795±89.6μm）。与对照饲料（0.021mm）相比，饲喂HY0.5、HY1.0和HY2.0饲料的罗非鱼绒毛平均表面积体积（SV）也更高（分别为0.025、0.026和0.038mm）。在HY1.0和HY2.0处理中，所有四个靶基因均显著上调。支持生长和ATP产生的基因，即胰岛素样生长因子-1（IGF-1）和甘油醛-3-磷酸（G-3-P）的表达显著改善，免疫相关基因hepcidin的表达也显著改善。随着HY水平的增加，胃饥饿素的表达也显著增加。可以得出结论，添加HY可提高尼罗罗非鱼的饲料利用率、肠道健康状况、营养吸收能力和免疫力。
