Wang Jianzhong, Liu Jing, Zhang Siyuan, Zhou Rui, Qiu Jicheng, Zhao Yi, Ma Xianglin, Wu Xiaojie, Li Xiaoguang, Mao Wei, Liu Yiduo
Shanxi Key Laboratory for Modernization of TCVM, College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong, China.
Beijing Yuanda Xinghuo Medicine Technology Co., Ltd., Beijing, China.
Front Vet Sci. 2025 Aug 25;12:1626094. doi: 10.3389/fvets.2025.1626094. eCollection 2025.
Canine babesiosis, caused by Babesia canis, is a tick-borne hemolytic disease requiring rapid, reliable diagnostic tools to protect canine health in resource-limited settings.
We developed a colloidal gold immunochromatographic assay (CGIA) strip using a recombinant BcMSA1-BcSA1 fusion protein, expressed in Escherichia coli with a yield of 2.5 mg/L, combining hydrophilic domains of merozoite surface antigen (BcMSA1) and secreted antigen (BcSA1). Seventy-two serum samples from veterinary clinics in Shanxi Province, China (ethical approval SXAU-2022-013), were tested against a commercial ELISA kit (Anigen Rapid B. canis Ab Test Kit), with infections confirmed by microscopy and PCR. Specificity was assessed using sera positive for Theileria spp., Toxoplasma gondii, Ancylostoma caninum, Eimeria canis, Canine distemper virus, and Canine parvovirus.
The CGIA strip showed no cross-reactivity, a detection limit of 1:8 for B. canis-positive sera, and retained efficacy after 18 months at room temperature. Sensitivity was 84%, specificity 93.6%, and Cohen's Kappa was 0.935 compared to ELISA.
This stable, user-friendly CGIA strip offers an efficient point-of-care solution for B. canis detection, overcoming limitations of traditional methods and supporting epidemiological and clinical applications.
由犬巴贝斯虫引起的犬巴贝斯虫病是一种蜱传播的溶血性疾病，在资源有限的环境中需要快速、可靠的诊断工具来保护犬类健康。
我们使用重组BcMSA1-BcSA1融合蛋白开发了一种胶体金免疫层析检测（CGIA）试纸条，该融合蛋白在大肠杆菌中表达，产量为2.5 mg/L，结合了裂殖子表面抗原（BcMSA1）和亲水结构域和分泌抗原（BcSA1）。来自中国山西省兽医诊所的72份血清样本（伦理批准号SXAU-2022-013），用商业ELISA试剂盒（Anigen Rapid B. canis Ab Test Kit）进行检测，通过显微镜检查和PCR确认感染情况。使用泰勒虫属、弓形虫、犬钩虫、犬艾美耳球虫、犬瘟热病毒和犬细小病毒阳性血清评估特异性。
CGIA试纸条无交叉反应，对犬巴贝斯虫阳性血清的检测限为1:8，在室温下保存18个月后仍保持效力。与ELISA相比，灵敏度为84%，特异性为93.6%，Cohen's Kappa为0.935。
这种稳定、用户友好的CGIA试纸条为犬巴贝斯虫检测提供了一种高效的即时检测解决方案，克服了传统方法的局限性，支持流行病学和临床应用。
