Leaves n-Hexane Fraction Antibacterial Activity and the Inhibition of Gene Expression of and in the Combination ATCC 25175 and ATCC 10790.

BACKGROUND

Oral biofilm is an extracellular polymeric matrix produced by as the early colonizer in the formation of an oral biofilm. Glucosyltransferase (Gtfs) enzyme is a virulence factor for in biofilm that is resistant to antibacterial agents. influences to increased quantities of Gtfs, thereby accelerating the growth of oral biofilms. Decreasing the production of Gtfs by the use of additional materials is necessary to inhibit biofilm formation.

OBJECTIVE

The study was determined the inhibitory activity of the leaves n-hexane fraction on the production of Gtfs by a single and combination of and

METHODS

Antibacterial effect of the leaves n-hexane fraction against and was examined by the inhibition zone and, and MBC. The antibiofilm was examined on the expression of and in , and their combination using qRT-PCR. The results were analyzed with Kruskal-Wallis test and Mann-Whitney -test.

RESULTS

leaves inhibited the growth of and at the lowest concentration (3.125%), with the inhibition zones are larger than chlorhexidine (CHX). The effect on the Gtfs activity shown the gene expression of remained unchanged in both single and combined species, while it was decreased in the single at a concentration of 0.78%, but itdoes not exhibit a significant difference between single and double species. Conversely, gene expression in the dual-species was reduced 90.43% by the n-hexane fraction at a concentration of 0.78%.

CONCLUSION

The leaves n-hexane fraction exhibited antibacterial activity against and . It may exhibit antibiofilm formation, as it could reduce the expression of the in the double species bacteria.