小韦荣球菌对变形链球菌生理活性的影响。
Effect of Veillonella parvula on the physiological activity of Streptococcus mutans.
机构信息
State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu 610041, China; Department of Cariology and Endodontics, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
Healthy Food Evaluation Research Center, West China School of Public Health, Sichuan University, Chengdu, 610041, China.
出版信息
Arch Oral Biol. 2020 Jan;109:104578. doi: 10.1016/j.archoralbio.2019.104578. Epub 2019 Sep 26.
OBJECTIVE
The aim of this study was to observe the effect of V. parvula on the physiological activity of S. mutans and elucidate the role of V. parvula on dental caries.
DESIGN
We constructed dual-species biofilms formed by V. parvula and S. mutans, and measured the pH dynamics, biofilm growth, Extracellular Polysaccharide (EPS) synthesis, and expression of S. mutans EPS synthesis-associated genes affected by V. parvula.
RESULTS
pH dynamics were not altered when V. parvula and S. mutans were co-cultured during a 120 -h test period. However, S. mutans cell number and EPS synthesis in dual-species biofilms were found to be significantly higher than in single-species biofilms. Moreover, expression levels of genes encoding glucosyltransferases (gtfs), gtfB and gtfC specifically, were up-regulated when S. mutans was co-cultured with V. parvula.
CONCLUSIONS
Our findings indicate that V. parvula is not, as previously thought, protective and associated with caries health. On the contrary, V. parvula might participate in caries development through interactions with S. mutans. This study suggests that V. parvula may have an impact on the pathogenesis of dental caries.
目的
本研究旨在观察小韦荣球菌对变形链球菌生理活性的影响，并阐明小韦荣球菌在龋齿中的作用。
设计
我们构建了由小韦荣球菌和变形链球菌组成的双菌种生物膜，并测量了 pH 动力学、生物膜生长、细胞外多糖（EPS）合成以及受小韦荣球菌影响的变形链球菌 EPS 合成相关基因的表达。
结果
在 120 h 的测试期内，当小韦荣球菌和变形链球菌共培养时，pH 动力学没有改变。然而，在双菌种生物膜中，变形链球菌的细胞数量和 EPS 合成明显高于单菌种生物膜。此外，当变形链球菌与小韦荣球菌共培养时，编码葡糖基转移酶（gtfs）、gtfB 和 gtfC 的基因表达水平上调。
结论
我们的研究结果表明，小韦荣球菌并非如先前认为的那样具有保护作用，与龋齿健康有关。相反，小韦荣球菌可能通过与变形链球菌的相互作用参与龋齿的发生。本研究提示小韦荣球菌可能对龋齿的发病机制有影响。
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