Avelar Ramon Ivo Soares, Mendes Marcelo Henrique Avelar, Muñoz de Páez Betsy Carolina, Araújo Ana Beatriz Silva, Resende Luciane Vilela, Carvalho Elisangela Elena Nunes, Cavalcanti Vytória Piscitelli, de Oliveira Cleiton Lourenço
Departament of Agriculture, Federal University of Lavras, Lavras, Minas Gerais CEP 37203-202, Brazil.
School of Agricultural Sciences of Lavras (ESAL), Federal University of Lavras, Lavras, Minas Gerais CEP 37203-202, Brazil.
ACS Omega. 2025 Aug 26;10(35):39589-39605. doi: 10.1021/acsomega.5c02517. eCollection 2025 Sep 9.
Multivariate analysis techniques can be useful for analyzing data that seeks to separate food plant cultivars according to yield, leaf quality and nutritional value. Thus, we used, validated and compared principal component analysis (PCA), Kohonen's organizable maps (SOM), a nonsupervised competitive learning artificial neural network formed by a grid of (artificial neurons) and multifactorial analysis to differentiate three cultivars of tree ), an unconventional food plant (PANC). For the differentiation, physicochemical variables of the leaves were evaluated in order to determine their sensory quality (total titratable acidity, total soluble solids, pH), antioxidant properties (vitamin C and total phenolics), nutritional and mineral composition (centesimal composition and macro and microelements), and yield results indicate that the highest leaf yield occurred in the first cutting for the three varieties evaluated, the nutritional quality of the leaves increased progressively as the experiment progressed, reaching significantly higher values in the third cut for some macro and micronutrients: Ca (278.9 g kg), Mg (64.1 9 g kg), S (2.53 g kg), Fe (1.78 g kg), Mn (1.93 mg 100 g). Among the varieties evaluated, SG stood out for presenting the best antioxidant properties, due to the highest concentration of ascorbic acid (355.18 mg 100 g) and total phenolics (569.2 mg GAE 100 g) in the second cut, along with a significantly higher average of free radical scavenging (SRL = 17.4%) when compared to PP and PR with values of 14.0 and 9.1%, respectively. According to the validation of the multivariate methods, all were suitable for analyzing the data obtained. SOM and PCA showed similar results, but PCA needed three components to be able to explain 75% of the data in a three-dimensional graph, while SOM made it possible to more efficiently explore the tendency to group the samples into seven groups according to the similarities and differences in the variables evaluated. This is due to its ability to reduce the size of the data and maintain a true representation of the relevant properties of the input vectors, generating a two-dimensional map that allows the results to be easily visualized.
多元分析技术对于分析旨在根据产量、叶片质量和营养价值来区分食用植物品种的数据可能很有用。因此,我们使用、验证并比较了主成分分析(PCA)、科霍宁自组织映射(SOM)(一种由(人工神经元)网格构成的无监督竞争学习人工神经网络)和多因素分析,以区分三种树品种,一种非常规食用植物(PANC)。为了进行区分,对叶片的理化变量进行了评估,以确定其感官质量(总可滴定酸度、总可溶性固形物、pH值)、抗氧化特性(维生素C和总酚类)、营养和矿物质组成(百分组成以及常量和微量元素),产量结果表明,在所评估的三个品种中,第一次刈割时叶片产量最高,随着实验的进行,叶片的营养质量逐渐提高,在第三次刈割时,一些常量和微量营养素达到了显著更高的值:钙(278.9克/千克)、镁(64.19克/千克)、硫(2.53克/千克)、铁(1.78克/千克)、锰(1.93毫克/100克)。在所评估的品种中,SG表现突出,具有最佳的抗氧化特性,因为在第二次刈割时,其抗坏血酸(355.18毫克/100克)和总酚类(569.2毫克没食子酸当量/100克)的浓度最高,与PP和PR相比,其平均自由基清除率(SRL = 17.4%)也显著更高,PP和PR的值分别为14.0%和9.1%。根据多元方法的验证,所有方法都适用于分析所获得的数据。SOM和PCA显示出相似的结果,但PCA需要三个成分才能在三维图中解释75%的数据,而SOM能够更有效地探索根据所评估变量的异同将样本分组为七组的趋势。这是由于它能够减小数据规模并保持输入向量相关属性的真实表示,生成一个二维图,使结果易于可视化。