Alim M A, Hossain M M K, Jahan N, Rubaya R, Bhuyan A A, Alam J, Ahammad I, Siddiki M S R, Moniruzzaman M
Animal Biotechnology Division, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka-1349, Bangladesh.
Department of Animal Nutrition, Genetics and Breeding, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka 1207, Bangladesh.
Iran J Vet Res. 2025;26(1):68-77. doi: 10.22099/ijvr.2025.49855.7351.
To meet up the demand gap of milk in Bangladesh, short-term, midterm, and long-term goal have been set up by the government through crossing with Bangladeshi local cattle and high-producing foreign cattle like Friesian, Jersey, Sahiwal, etc.
The purpose of this study was to identify the single nucleotide polymorphisms (SNPs) in the gene, to check the structural and functional impact of mutant proteins on milk production traits that are significantly associated.
Four SNPs were identified in exons 26, 36, 38, and 41 of the gene using pooled DNA sequencing, but only one SNP g.17924 A>G was a non-synonymous that changed the amino acid threonine to alanine in the FASN protein and the other three SNPs were silent mutations. Structural and functional prediction analysis were done with a series of techniques to detect remote protein homology and predict structures, structural integrity, structure quality, protein stability, protein motion, flexibility, and stability impact, conservation profile and finally molecular dynamics simulations for wild-type and mutant protein expression differences.
The non-synonymous g.17924 A>G mutation showed a clear difference between wild and mutant proteins, indicating the impact on the observed phenotype. Then, SNP g.17924 A>G was genotyped in 100 milking cows aiming to check the association effects. SNP g.17924 A>G was found to have significant allele substitution effects on milk yield traits.
Our results suggest that the identified polymorphism affects milk yield traits in the studied population and could be used as genetic marker for cattle selection processes aiming to increase productivity.
为了弥补孟加拉国牛奶的需求缺口，政府通过将孟加拉国本地牛与高产外国牛（如弗里生牛、泽西牛、 sahiwal 牛等）杂交，制定了短期、中期和长期目标。
本研究的目的是鉴定该基因中的单核苷酸多态性（SNP），检查突变蛋白对显著相关的牛奶生产性状的结构和功能影响。
使用混合 DNA 测序在该基因的外显子 26、36、38 和 41 中鉴定出四个 SNP，但只有一个 SNP g.17924 A>G 是非同义突变，它将 FASN 蛋白中的苏氨酸氨基酸改变为丙氨酸，其他三个 SNP 是沉默突变。使用一系列技术进行结构和功能预测分析，以检测远程蛋白质同源性并预测结构、结构完整性、结构质量、蛋白质稳定性, 蛋白质运动、灵活性和稳定性影响、保守概况，最后进行野生型和突变型蛋白质表达差异的分子动力学模拟。
非同义 g.17924 A>G 突变在野生型和突变型蛋白质之间显示出明显差异，表明对观察到的表型有影响。然后，对 100 头奶牛进行 SNP g.17924 A>G 基因分型，以检查关联效应。发现 SNP g.17924 A>G 对牛奶产量性状有显著的等位基因替代效应。
我们的结果表明，所鉴定的多态性影响研究群体中的牛奶产量性状，可作为旨在提高生产力的牛选择过程的遗传标记。
