Zhang Xiancui, Liao Haoran, Cai Tong, Cai Peiwen, Wu Xiangyu, Wang Zhe, Ma Haoyu, Qiu Guoqiang, Zhao Mingxing, Lu Xingmeng, Wang Xianting, Wu Choufei
School of Life Sciences, Huzhou University, Huzhou, China.
Shandong Center for Disease Control and Prevention, Jinan, China.
Front Microbiol. 2025 Aug 29;16:1652881. doi: 10.3389/fmicb.2025.1652881. eCollection 2025.
The lactic acid bacteria (LAB) has shown great potential as a sustainable solution to support agriculture through its plant-growth-promoting and biocontrol activities. However, their efficacy as bioinoculants is limited by unpredictable colonization in natural conditions.
The bacterial strain LP0308, identified as (LP0308) based on 16S rRNA sequence analysis, was obtained from rhizosphere soil. The features and colonization strategies of LP0308 were characterized through genome sequencing, tomato seed germination assays, pot experiments, and measurements of soil physicochemical properties and enzyme activities.
LP0308 was introduced into the soil of tomato, and it could stably persist and proliferate for a long-term (0-20 days), as confirmed by colony-forming unit (CFU), quantitative real-time PCR (RT-qPCR), and fluorescence hybridization (FISH) analyses. Further characterization revealed that LP0308 altered the microbial composition of the rhizosphere soil and significantly increased the abundance of and potentially pathogenic microorganism. Further analyses revealed that LP0308 altered the rhizosphere soil microbial community, significantly increasing the abundance of spp. while decreasing the potential pathogenic microorganisms, such as and . In addition, the successful colonization of LP0308 led to drastically increased expression of encoding biofilm (1, 2, , and 3), immune modulation (, , and ), and antimicrobial activity gene (). strain LP0308 was confirmed as a possible plant growth-promoting rhizobacteria (PGPR), which significantly promoted bud length, plant height, primary root length, root fresh weight, and whole-seedling fresh weight. Additionally, application of LP0308 markedly improved soil nutrient availability and stimulated key enzymatic activities.
Together, our findings suggest the LP0308 as a potential target for developing more effective bioinoculants for sustainable agriculture.
乳酸菌作为一种可持续的解决方案，通过促进植物生长和生物防治活动，在支持农业方面显示出巨大潜力。然而，它们作为生物接种剂的功效受到自然条件下不可预测的定殖的限制。
基于16S rRNA序列分析鉴定为（LP0308）的细菌菌株LP0308，从根际土壤中获得。通过基因组测序、番茄种子发芽试验、盆栽试验以及土壤理化性质和酶活性的测量，对LP0308的特征和定殖策略进行了表征。
将LP0308引入番茄土壤中，通过菌落形成单位（CFU）、定量实时PCR（RT-qPCR）和荧光原位杂交（FISH）分析证实，它可以长期（0 - 20天）稳定地存活和增殖。进一步表征表明，LP0308改变了根际土壤的微生物组成，并显著增加了 以及潜在致病微生物的丰度。进一步分析表明，LP0308改变了根际土壤微生物群落，显著增加了 属的丰度，同时减少了潜在致病微生物，如 和 。此外，LP0308的成功定殖导致编码生物膜（1、2、 和3）、免疫调节（ 、 和 ）和抗菌活性基因（ ）的表达大幅增加。菌株LP0308被确认为一种可能的促植物生长根际细菌（PGPR），它显著促进了芽长、株高、主根长度、根鲜重和全株鲜重。此外，LP0308的应用显著提高了土壤养分有效性，并刺激了关键酶活性。
总之，我们的研究结果表明LP0308是开发更有效的可持续农业生物接种剂的潜在目标。
