Li Yan, Gu Meiying, Xu Wanli, Zhu Jing, Chu Min, Tang Qiyong, Yi Yuanyang, Zhang Lijuan, Li Pan, Zhang Yunshu, Ghenijan Osman, Zhang Zhidong, Li Ning
College of Resource and Environment, Xinjiang Agricultural University, Urumqi 830052, China.
Xinjiang Laboratory of Special Environmental Microbiology, Institute of Microbiology, Xinjiang Uygur Autonomous Region Academy of Agricultural Sciences, Urumqi 830091, China.
Microorganisms. 2025 Jul 30;13(8):1781. doi: 10.3390/microorganisms13081781.
Soil salinity adversely affects crop growth and development, leading to reduced soil fertility and agricultural productivity. The indigenous salt-tolerant plant growth-promoting rhizobacteria (PGPR), as a sustainable microbial resource, do not only promote growth and alleviate salt stress, but also improve the soil microecology of crops. The strain H5 isolated from saline-alkali soil in Bachu of Xinjiang was studied through whole-genome analysis, functional annotation, and plant growth-promoting, salt-tolerant trait gene analysis. Phylogenetic tree analysis and 16S rDNA sequencing confirmed its classification within the genus . Functional annotation revealed that the H5 genome harbored multiple functional gene clusters associated with plant growth promotion and salt tolerance, which were critically involved in key biological processes such as bacterial survival, nutrient acquisition, environmental adaptation, and plant growth promotion. The pot experiment under moderate salt stress demonstrated that seed inoculation with sp. H5 not only significantly improved the agronomic traits of tomato seedlings, but also increased plant antioxidant enzyme activities under salt stress. Additionally, soil analysis revealed H5 treatment significantly decreased the total salt (9.33%) and electrical conductivity (8.09%), while significantly improving organic matter content (11.19%) and total nitrogen content (10.81%), respectively ( < 0.05). Inoculation of strain H5 induced taxonomic and functional shifts in the rhizosphere microbial community, increasing the relative abundance of microorganisms associated with plant growth-promoting and carbon and nitrogen cycles, and reduced the relative abundance of the genera (15.14%) and (9.76%), which are closely related to tomato diseases ( < 0.05). Overall, this strain exhibits significant potential in alleviating abiotic stress, enhancing growth, improving disease resistance, and optimizing soil microecological conditions in tomato plants. These results provide a valuable microbial resource for saline soil remediation and utilization.
土壤盐渍化对作物生长发育产生不利影响，导致土壤肥力和农业生产力下降。本地耐盐植物促生根际细菌（PGPR）作为一种可持续的微生物资源，不仅能促进作物生长、缓解盐胁迫，还能改善作物的土壤微生态。通过全基因组分析、功能注释以及植物促生长和耐盐性状基因分析，对从新疆巴楚盐碱土中分离出的菌株H5进行了研究。系统发育树分析和16S rDNA测序确定了其在该属内的分类。功能注释显示，H5基因组含有多个与植物促生长和耐盐相关的功能基因簇，这些基因簇在细菌存活、养分获取、环境适应和植物促生长等关键生物学过程中起着至关重要的作用。中度盐胁迫下的盆栽试验表明，用H5菌株接种种子不仅显著改善了番茄幼苗的农艺性状，还提高了盐胁迫下植株的抗氧化酶活性。此外，土壤分析表明，H5处理显著降低了总盐含量（9.33%）和电导率（8.09%），同时分别显著提高了有机质含量（11.19%）和总氮含量（10.81%）（P<0.05）。接种H5菌株引起了根际微生物群落的分类和功能变化，增加了与植物促生长以及碳氮循环相关微生物的相对丰度，降低了与番茄病害密切相关的属（15.14%）和属（9.76%）的相对丰度（P<0.05）。总体而言，该菌株在缓解番茄非生物胁迫、促进生长、提高抗病性和优化土壤微生态条件方面具有显著潜力。这些结果为盐碱土修复和利用提供了宝贵的微生物资源。
