Cardoso-Cardenas Mary, Bauer-Estrada Katherine, Diaz-Barrera Luis Eduardo, Quintanilla-Carvajal Maria Ximena
Agroindustrial Processes Research Group, Universidad de La Sabana, Engineering Department, Chía, Colombia.
Unisabana Center for Translational Science, Universidad de La Sabana, School of Medicine, Chía, Colombia.
Front Microbiol. 2025 Aug 29;16:1622211. doi: 10.3389/fmicb.2025.1622211. eCollection 2025.
Members of the Lactobacillaceae family have been extensively investigated for their health-promoting properties, including the restoration of cellular functions, inhibition of pathogen colonization, and cholesterol-lowering effects.
This study evaluated the hypocholesterolemic activity of K73 at various stages of encapsulation using three distinct capsule types, including simulated gastrointestinal (GI) transit and analysis of adhesion to HT-29 and RKO intestinal epithelial cells.
The strain demonstrated a pronounced ability to deconjugate taurine-conjugated bile salts, particularly taurocholic acid sodium (TCA) and sodium taurodeoxycholate acid (TDCA), as evidenced by viable cell counts. In bioreactor samples, counts reached 8.31 and 7.82 log CFU/mL for TCA and TDCA, respectively. After capsule dissolution, viability decreased across all formulations: C1 showed 6.90 and 5.04, C2 reached 4.99 and 4.68, and C3 recorded 6.10 and 4.12 log CFU/mL. Following digestion, C1 exhibited notable recovery, with 7.30 log CFU/mL (TCA) and 4.26 log CFU/mL (TDCA). Cholesterol absorption was highest in digested C1 (65.49%), surpassing the 24-h broth culture (63.9%). Adhesion to epithelial cells varied by formulation; for HT-29 cells, C1, C2, and C3 showed 21, 5, and 33 adhered bacteria, respectively, while for RKO cells, adhesion was 44 (C1), 22 (C2), and 201 (C3).
These findings demonstrate that K73 maintained its viability, hypocholesterolemic activity, and epithelial adhesion capacity throughout encapsulation and simulated GI digestion, supporting its potential application as a functional probiotic strain in nutraceutical and food products.
乳杆菌科成员因其促进健康的特性而受到广泛研究，包括恢复细胞功能、抑制病原体定植和降低胆固醇的作用。
本研究使用三种不同的胶囊类型，评估了K73在包封各个阶段的降胆固醇活性，包括模拟胃肠道（GI）转运以及对HT - 29和RKO肠上皮细胞的黏附分析。
该菌株表现出显著的能力去结合牛磺酸结合型胆汁盐，尤其是牛磺胆酸钠（TCA）和牛磺脱氧胆酸钠（TDCA），活菌计数证明了这一点。在生物反应器样品中，TCA和TDCA的计数分别达到8.31和7.82 log CFU/mL。胶囊溶解后，所有配方的活力均下降：C1显示为6.90和5.04，C2达到4.99和4.68，C3记录为6.10和4.12 log CFU/mL。消化后，C1表现出显著的恢复，TCA为7.30 log CFU/mL，TDCA为4.26 log CFU/mL。消化后的C1中胆固醇吸收最高（65.49%），超过24小时肉汤培养（63.9%）。对上皮细胞的黏附因配方而异；对于HT - 29细胞，C1、C2和C3分别显示有21、5和33个黏附细菌，而对于RKO细胞，黏附数分别为44（C1）、22（C2）和201（C3）。
这些发现表明，K73在整个包封和模拟胃肠道消化过程中保持了其活力、降胆固醇活性和上皮黏附能力，支持其作为功能性益生菌菌株在营养保健品和食品中的潜在应用。
